Work with the Experts in Biophysics

Our mission at 2bind is to accelerate your research by developing and performing robust, high-end, scalable, and cost-efficient biophysical assays for:

Drug Discovery

Fragment-based Drug Discovery

RNA-targeted Drug Discovery

Protein Interactions and Stability

Antibody Development

Aptamer Research

Services for Drug Discovery

High-throughput screening assay development
In-house compound libraries (5 000 and 150 000 library sizes)
High-throughput compound handling (LabCyte Echo platform)
High-throughput biophysical screening (MST, Dianthus, nanoDSF)
Hit-Validation (MST, Dianthus, nanoDSF, BLI, and ITC based hit confirmation assays)
Hit-Characterization (in-depth analysis of lead affinity, kinetics, thermodynamics, stoichiometry, influence of bioliquids)
In silico pre-screening of up to 3 Million compounds
Integrated drug discovery platform (from hit ID to IND filing)

Services for Fragment-based Drug Discovery

High-throughput screening assay development
In-house libraries (2 000 and 3 600 library sizes)
High-throughput fragment handling (LabCyte Echo platform)
High-throughput biophysical screening (MST, Dianthus, nanoDSF)
Hit-Validation (MST, Dianthus, nanoDSF, BLI, and ITC based hit confirmation assays)
In silico pre-screening of fragments
Integrated drug discovery program (from fragment ID to IND filing)

Services for RNA-targeted Drug Discovery

High-throughput screening assay development
In-house compound libraries (5 000 and 150 000 library sizes)
In-house fragment libraries (~ 2000 library size)
High-throughput compound handling (LabCyte Echo platform)
High-throughput biophysical screening (MST, Dianthus)
Hit-Validation (MST, Dianthus, BLI, and ITC based hit confirmation assays)
Hit-Characterization (in-depth analysis of lead affinity, kinetics, thermodynamics, stoichiometry, influence of bioliquids)

Services for Protein Interaction Analysis

Steady-state binding assay (affinity, K_D), MST, nanoDSF, BLI, ITC
High-throughput binding screening (MST, nanoDSF)
Kinetic binding assays (BLI)
Thermodynamic profiling (ITC; binding enthalpy and entropy, free enthalpy of binding, rection stoichiometry)
Assays in bioliquids (bio-availability, off-target effects)
Competition assays (ligand displacement, complex formation)
Thermal-shift binding assays

Services for Protein Stability Analysis

Buffer screening and optimization (nanoDSF; custom or pre-made buffers, 96-well buffer plates available)
Long-term storage optimization (quality control)
Protein batch comparison
Forced-degradation assays

Services for Antibody-Target Interactions

Steady-state binding assay (affinity, K_D), MST, BLI, ITC
Kinetic binding assays (BLI)
Thermodynamic profiling (ITC; binding enthalpy and entropy, free enthalpy of binding, rection stoichiometry)
Assays in bioliquids (bio-availability, off-target effects)
Epitope binning (discrimination between different epitopes on one antigen)
Antibody quantitation in culture supernatants

Services for Antibody Stability and Aggregation

Buffer screening and optimization (nanoDSF; custom or pre-made buffers, 96-well buffer plates available)
Antibody pre-formulation development (stability analysis of antibodies in 96 buffer conditions from FDA- and EMA-approved therapeutic antibodies)
Long-term storage optimization (quality control)
Biosimilar comparison
Forced-degradation assays
Deep-feature, mutation, and glycosylation analysis

Services for Aptamer Research

Steady-state binding assay (affinity, K_D, MST, BLI, ITC)
Kinetic binding assays (BLI)
High-throughput screening assays (ligand binding screen, MST)
Thermodynamic profiling (ITC; binding enthalpy and entropy, free enthalpy of binding, rection stoichiometry)
Assays in bioliquids (bio-availability, off-target effects)
Sandwich assays (Interaction site mapping)
Competition assays

Our Mindset - Working with us

2bind is one of the world’s leading service providers for biophysics-based assays. We have worked with 6 of the top 10 pharmaceutical companies world-wide – e.g. Roche – and with many top-tier Universities – e.g. MIT and Stanford. Just as well, many SMEs and life-science start-ups trust us with their biophysical assay projects.

Milestone-based projects

Milestones and exit-options give you the full control over your projects.

Clear cost-structure

All costs are known prior to project start. Cancelled milestones are not charged.

Fast turn-around times

The structured project pipeline ensures turn-around times of few working days even for complex screening projects.

Long-standing expertise

2bind is the first CRO certified by NanoTemper for MST and nanoDSF.

Personal project manager

All customer projects are handled by highly skilled, PhD-level biochemists and biophysicists.

Customers from Pharma, Biotech, and Academia

Completed contract research projects

Years of experience with MST and nanoDSF

Protein, DNA, RNA targets analyzed

State-of-the-art Biophysics

Our state-of-the-art portfolio of biophysical technologies enables us to provide you with a complete and comprehensive picture of your specific question: From affinity and stability to kinetics and thermodynamics.

K_D | EC₅₀ | T_m | ΔT_m | T_agg | ΔT_agg | k_on | k_off | n | ΔH | ΔG | ΔS

MST

Steady-state affinity
Ligand binding
Screening
Drug discovery
Proteins, DNA, RNA
Bioliquids

BLI

Kinetics
Ligand binding
Antibody development
Off-rate ranking
Bioliquids

Dianthus

Steady-state affinity
Ligand binding
Screening
Drug discovery
Proteins, DNA, RNA
Bioliquids

ITC

Thermodynamics
Stoichiometry
Ligand binding

nanoDSF

Protein stability
Protein unfolding & aggregation
Ligand binding
Screening
Drug discovery
Bioliquids

LabCyte Echo

Compound handling
Fragment handling
Industry-standard dilution series

Clients & Partners

We used the services of 2bind several times, including MST, BLI and nanoDSF and were always happy with the excellent service and result quality.

Philipp Richle CDR-Life AG

We are impressed with the rapid turnaround time and excellent service, as well the high quality data generated at 2bind.

Brian Hamman, Director of Biophysics Arvinas, Inc.

The [2bind] assay development was well-planned, careful and methodical. Progress reports were frequent, very clear and meticulously prepared. Their science and professionalism are impeccable – working with 2bind is like having expert biophysicists in the lab next door!

Chris Lepre, PhD, Biochemistry, Biophysics and Structural Biology Relay Therapeutics, Inc.

The MST experts at 2bind were successfull in setting up various binding assays, stoichiometry assays, and competition assays and helped us to drive our science. We are very satisfied with the low sample consumption and the fast turnaround time.

Mads Nøregaard-Madsen, Chief Innovation & Information Officer Nuevolution

During several years we have had excellent experience working with 2bind. They have always provided us with the highest-quality services. Quick responsiveness of 2bind’s team has helped us to accelerate projects.
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Dmitry Shishov Gero

We have enjoyed the quality of service of 2bind coupled to a great flexibility. 2bind’s technology allowed us to quickly assess target validation with dozens of proteins and helped us advance quickly on the subject.
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Philippe Verwaerde, President and CSO AlzProtect