Our mission at 2bind is to accelerate your research by developing and performing robust, high-end, scalable, and cost-efficient biophysical assays for:
Drug Discovery
Fragment-based Drug Discovery
RNA-targeted Drug Discovery
Protein Interactions and Stability
Antibody Development
Aptamer Research
Services for Drug Discovery
- High-throughput screening assay development
- In-house compound libraries (5 000 and 150 000 library sizes)
- High-throughput compound handling (LabCyte Echo platform)
- High-throughput biophysical screening (MST, Spectral Shift, GCI, Dianthus, nanoDSF)
- Hit-Validation (MST, Spectral Shift, GCI, Dianthus, nanoDSF, BLI, and ITC based hit confirmation assays)
- Hit-Characterization (in-depth analysis of lead affinity, kinetics, thermodynamics, stoichiometry, influence of bioliquids)
- In silico pre-screening of up to 3 Million compounds
- Integrated drug discovery platform (from hit ID to IND filing)
Services for Fragment-based Drug Discovery
- High-throughput screening assay development
- In-house fragment libraries (2 000 and 3 600 library sizes)
- High-throughput fragment handling (LabCyte Echo platform)
- High-throughput biophysical screening (MST, Spectral Shift, GCI, Dianthus, nanoDSF)
- Hit-Validation (MST, Spectral Shift, GCI, Dianthus, nanoDSF, and ITC based hit confirmation assays)
- In silico pre-screening of fragments
- Integrated drug discovery program (from fragment ID to IND filing)
Services for RNA-targeted Drug Discovery
- High-throughput screening assay development
- In-house compound libraries (5 000 and 150 000 library sizes)
- In-house fragment libraries (~ 2000 library size)
- High-throughput compound handling (LabCyte Echo platform)
- High-throughput biophysical screening (MST, Spectral Shift, Dianthus, GCI)
- Hit-Validation (MST, Spectral Shift, GCI, BLI, and ITC based hit confirmation assays)
- Hit-Characterization (in-depth analysis of lead affinity, kinetics, thermodynamics, stoichiometry, influence of bioliquids)
Services for Protein Interaction Analysis
- Steady-state binding assay (affinity, KD), MST, nanoDSF, BLI, ITC
- High-throughput binding screening (MST, nanoDSF)
- Kinetic binding assays (BLI)
- Thermodynamic profiling (ITC; binding enthalpy and entropy, free enthalpy of binding, rection stoichiometry)
- Assays in bioliquids (bio-availability, off-target effects)
- Competition assays (ligand displacement, complex formation)
- Thermal-shift binding assays
Services for Protein Stability Analysis
- Buffer screening and optimization (nanoDSF; custom or pre-made buffers, 96-well buffer plates available)
- Long-term storage optimization (quality control)
- Protein batch comparison
- Forced-degradation assays
Colloidal, Thermal, Chemical Stability
- High-throughput developability screens
- Buffer compatibility screens
- In-depth characterization
- Long-term storage stability
- Stress tests
- Antibody pre-formulation
- Forced-degradation assays
- QC
- Batch release
Affinity, Avidity, Quantification
- High-throughput affinity/avidity screens
- Early-stage clone ranking (kon, koff, KD)
- Antibody quantitation in cell culture supernatants
- Avidity screens of immune sera
- In-depth characterization of binding affinity and avidity
- Epitope binning
- Off-target screens
- Real-time kinetics on target cells
Services for Aptamer Research
- Steady-state binding assay (affinity, KD, MST, Spectral Shift, Dianthus, GCI, ITC)
- Kinetic binding assays (GCI, heliX, BLI)
- High-throughput screening assays (ligand binding screen, MST, Spectral Shift, GCI)
- Thermodynamic profiling (ITC; binding enthalpy and entropy, free enthalpy of binding, rection stoichiometry)
- Assays in bioliquids (bio-availability, off-target effects)
- Sandwich assays (Interaction site mapping)
- Competition assays
Our Mindset - Working with us
2bind is one of the world’s leading service providers for biophysics-based assays. We have worked with 6 of the top 10 pharmaceutical companies world-wide – e.g. Roche – and with many top-tier Universities – e.g. MIT and Stanford. Just as well, many SMEs and life-science start-ups trust us with their biophysical assay projects.
Milestone-based projects
Milestones and exit-options give you the full control over your projects.
Clear cost-structure
All costs are known prior to project start. Cancelled milestones are not charged.
Fast turn-around times
The structured project pipeline ensures turn-around times of few working days even for complex screening projects.
Long-standing expertise
2bind is the first CRO certified by NanoTemper for MST and nanoDSF.
Personal project manager
All customer projects are handled by highly skilled, PhD-level biochemists and biophysicists.
Customers from Pharma, Biotech, and Academia
Completed contract research projects
Years of experience with MST and nanoDSF
Protein, DNA, RNA targets analyzed
State-of-the-art Biophysics
Our cutting-edge portfolio of biophysical technologies provides a comprehensive answer to your question:
From binding to affinity.
From stability and folding to formulation.
From kinetics to thermodynamics.
KD | EC50 | Tm | ΔTm | Tagg | ΔTagg | kon | koff | n | ΔH | ΔG | ΔS | rh | PDI | Tsize | kD
Automated MST
APPLICATION AREAS
- Drug discovery
- Fragment screening
- Compound screening
- Protein interaction analysis
PARAMETERS
- Single-dose binding
- Steady-state affinity (KD)
NANOTEMPER CERTIFIED SERVICE PROVIDER
Dianthus
APPLICATION AREAS
- Drug discovery
- Fragment screening
- Compound screening
PARAMETERS
- Single-dose binding
- Steady-state affinity (KD)
NANOTEMPER CERTIFIED SERVICE PROVIDER
MST
APPLICATION AREAS
- Small-scale interaction analysis
- Assay development
- Aptamer binding
- Protein-protein interaction analysis
- Competition assays
PARAMETERS
- Single-dose binding
- Steady-state affinity (KD)
NANOTEMPER CERTIFIED SERVICE PROVIDER
GCI - WAVE
APPLICATION AREAS
- Drug discovery
- Fragment screening
- Compound screening
- Off-rate ranking
- Biologics development
PARAMETERS
- Binding kinetics (kon, ka, koff, kd)
- Steady-state affinity (KD)
- Enthalpy (ΔH)
CREOPTIX CERTIFIED SERVICE PROVIDER
heliX biosensor
APPLICATION AREAS
- Broad-range kinetic binding
- Molecular conformation
- Multi-specific binders
- PROTACs
- Nucleic acids targeting
- Enzymatic activity
PARAMETERS
- Binding kinetics (kon, ka, koff, kd)
- Steady-state affinity (KD)
- Conformation
- Ternary complex formation
DBS-CERTIFIED SERVICE PROVIDER
Spectral Shift
APPLICATION AREAS
- Small-scale interaction analysis
- Assay development
- Aptamer binding
- Protein-protein interaction analysis
- Competition assays
PARAMETERS
- Single-dose binding
- Steady-state affinity (KD)
NANOTEMPER CERTIFIED SERVICE PROVIDER
nanoDSF
APPLICATION AREAS
- Protein stability and unfolding analysis
- Buffer screening and optimization
- Protein aggregation analysis
- Pre- and formulation development
- Ligand binding (thermal shift)
PARAMETERS
- Protein unfolding (Tm, Tonset, Tturbidity, kd)
- Binding (Tm-diff, EC50)
NANOTEMPER CERTIFIED SERVICE PROVIDER
DLS - Panta
APPLICATION AREAS
- Protein quality control
- Particle size determination
- Solutio homogeneity
- Protein aggregation analysis
- Pre- and formulation development
- Buffer screening and optimization
PARAMETERS
- Paticle parameters (rh, PDI)
- Aggregation analysis (Tsize, Tscattering)
NANOTEMPER CERTIFIED SERVICE PROVIDER
DLS - Wyatt
APPLICATION AREAS
- Protein quality control
- Particle size determination
- Compound solubility
- Solutio homogeneity
- Protein aggregation analysis
- Pre- and formulation development
- Buffer screening and optimization
- DoE setups
PARAMETERS
- Paticle parameters (rh, PDI, MW)
- Aggregation analysis (Tsize, Tscattering)
WYATT-CERTIFIED SERVICE PROVIDER
BLI
APPLICATION AREAS
- Biologics development
- Antibody-antigen interaction analysis
- Off-rate ranking
PARAMETERS
- Binding kinetics (kon, ka, koff, kd)
- Steady-state affinity (KD)
- Enthalpy (ΔH)
ITC
APPLICATION AREAS
- Thermodynamic interaction analysis
- Drug discovery
- Compound and fragment binding
- Protein-protein interaction analysis
PARAMETERS
- Steady-state affinity (KD)
- Binding stoichiometry (n)
- Binding thermodynamics (ΔH, ΔS, ΔG)
LabCyte Echo
APPLICATION AREAS
- Compound spotting/printing
- Fragment spotting/printing
- Contact-free, high-precision dilution series
- Industry-standard
HPLC Analytics
APPLICATION AREAS
- Antibody and protein characterization
- Antibody-antigen interaction analysis
- Off-rate ranking
PARAMETERS
- Molecular size, weight (kDa), fragmentation, oligomeric state, aggregation, complex stoichiometry via SEC-MALS
- Hydrophobicity via HIC (Hydrophobic Interaction Chromatography)
- Charge state and variants via IEX (Ion-Exchange Chromatography)
Clients & Partners
Assay development, quantitative biology, high-throughput screening, in vitro biactivity, lab automation.
NanoTemper-certified service provider for MST and nanoDSF. Collaboration in beta-testing or instruments and consumables.
Creoptix-certified service provider for GCI/WAVE kinetic analysis. Collaboration in beta-testing or instruments and consumables.
AI-based chemical library screening, protein off-target prediction, chemical hit diversification.
Integrated DRIVE™ drug discovery and development platform.
Lead compound selection, mass spectrometry-based on- and off-target analysis and target deconvolution.
GMP-compliant production of recombinant proteins, cell line development.
heliX® biosensor and switchSENSE® technology, cooperation for assay and method development.
Structure-guided hit validation with proprietary crystallization platform.
Find out more about our partners and the 2bind Analytics Service Network here!