Work with the Experts in Biophysics

2bind joines the DRIVE platform: Your tool for fully integrated drug discovery. Learn more here.

Our mission at 2bind is to accelerate your research by developing and performing robust, high-end, scalable, and cost-efficient biophysical assays for:

Drug Discovery

Fragment-based Drug Discovery

RNA-targeted Drug Discovery

Protein Interactions and Stability

Antibody Development

Aptamer Research

Services for Drug Discovery

  • High-throughput screening assay development
  • In-house compound libraries (5 000 and 150 000 library sizes)
  • High-throughput compound handling (LabCyte Echo platform)
  • High-throughput biophysical screening (MST, Dianthus, nanoDSF)
  • Hit-Validation (MST, Dianthus, nanoDSF, BLI, and ITC based hit confirmation assays)
  • Hit-Characterization (in-depth analysis of lead affinity, kinetics, thermodynamics, stoichiometry, influence of bioliquids)
  • In silico pre-screening of up to 3 Million compounds
  • Integrated drug discovery platform (from hit ID to IND filing)

Services for Fragment-based Drug Discovery

  • High-throughput screening assay development
  • In-house libraries (2 000 and 3 600 library sizes)
  • High-throughput fragment handling (LabCyte Echo platform)
  • High-throughput biophysical screening (MST, Dianthus, nanoDSF)
  • Hit-Validation (MST, Dianthus, nanoDSF, BLI, and ITC based hit confirmation assays)
  • In silico pre-screening of fragments
  • Integrated drug discovery program (from fragment ID to IND filing)

Services for RNA-targeted Drug Discovery

  • High-throughput screening assay development
  • In-house compound libraries (5 000 and 150 000 library sizes)
  • In-house fragment libraries (~ 2000 library size)
  • High-throughput compound handling (LabCyte Echo platform)
  • High-throughput biophysical screening (MST, Dianthus)
  • Hit-Validation (MST, Dianthus, BLI, and ITC based hit confirmation assays)
  • Hit-Characterization (in-depth analysis of lead affinity, kinetics, thermodynamics, stoichiometry, influence of bioliquids)

Services for Protein Interaction Analysis

  • Steady-state binding assay (affinity, KD), MST, nanoDSF, BLI, ITC
  • High-throughput binding screening (MST, nanoDSF)
  • Kinetic binding assays (BLI)
  • Thermodynamic profiling (ITC; binding enthalpy and entropy, free enthalpy of binding, rection stoichiometry)
  • Assays in bioliquids (bio-availability, off-target effects)
  • Competition assays (ligand displacement, complex formation)
  • Thermal-shift binding assays

Services for Protein Stability Analysis

  • Buffer screening and optimization (nanoDSF; custom or pre-made buffers, 96-well buffer plates available)
  • Long-term storage optimization (quality control)
  • Protein batch comparison
  • Forced-degradation assays

Services for Antibody-Target Interactions

  • Steady-state binding assay (affinity, KD), MST, BLI, ITC
  • Kinetic binding assays (BLI)
  • Thermodynamic profiling (ITC; binding enthalpy and entropy, free enthalpy of binding, rection stoichiometry)
  • Assays in bioliquids (bio-availability, off-target effects)
  • Epitope binning (discrimination between different epitopes on one antigen)
  • Antibody quantitation in culture supernatants

Services for Antibody Stability and Aggregation

  • Buffer screening and optimization (nanoDSF; custom or pre-made buffers, 96-well buffer plates available)
  • Antibody pre-formulation development (stability analysis of antibodies in 96 buffer conditions from FDA- and EMA-approved therapeutic antibodies)
  • Long-term storage optimization (quality control)
  • Biosimilar comparison
  • Forced-degradation assays
  • Deep-feature, mutation, and glycosylation analysis

Services for Aptamer Research

  • Steady-state binding assay (affinity, KD, MST, BLI, ITC)
  • Kinetic binding assays (BLI)
  • High-throughput screening assays (ligand binding screen, MST)
  • Thermodynamic profiling (ITC; binding enthalpy and entropy, free enthalpy of binding, rection stoichiometry)
  • Assays in bioliquids (bio-availability, off-target effects)
  • Sandwich assays (Interaction site mapping)
  • Competition assays 

Our Mindset - Working with us

2bind is one of the world’s leading service providers for biophysics-based assays. We have worked with 6 of the top 10 pharmaceutical companies world-wide – e.g. Roche – and with many top-tier Universities – e.g. MIT and Stanford. Just as well, many SMEs and life-science start-ups trust us with their biophysical assay projects.

Milestone-based projects

Milestones and exit-options give you the full control over your projects.

Clear cost-structure

All costs are known prior to project start. Cancelled milestones are not charged.

Fast turn-around times

The structured project pipeline ensures turn-around times of few working days even for complex screening projects.

Long-standing expertise

2bind is the first CRO certified by NanoTemper for MST and nanoDSF.

Personal project manager

All customer projects are handled by highly skilled, PhD-level biochemists and biophysicists.

Customers from Pharma, Biotech, and Academia

Completed contract research projects

Years of experience with MST and nanoDSF

Protein, DNA, RNA targets analyzed

State-of-the-art Biophysics

Our state-of-the-art portfolio of biophysical technologies enables us to provide you with a complete and comprehensive picture of your specific question: From affinity and stability to kinetics and thermodynamics.

KD | EC50 | Tm | ΔTm | Tagg | ΔTagg | kon | koff | n | ΔH | ΔG | ΔS

MST

  • Steady-state affinity
  • Ligand binding
  • Screening
  • Drug discovery 
  • Proteins, DNA, RNA
  • Bioliquids

BLI

  • Kinetics
  • Ligand binding
  • Antibody development
  • Off-rate ranking
  • Bioliquids

Dianthus

  • Steady-state affinity
  • Ligand binding
  • Screening
  • Drug discovery 
  • Proteins, DNA, RNA
  • Bioliquids

ITC

  • Thermodynamics
  • Stoichiometry
  • Ligand binding

nanoDSF

  • Protein stability
  • Protein unfolding & aggregation
  • Ligand binding
  • Screening
  • Drug discovery
  • Bioliquids

LabCyte Echo

  • Compound handling
  • Fragment handling
  • Industry-standard dilution series

Clients & Partners

Working with 2bind was a great experience. As a deep tech company, collaborations with specialists are important to navigate complex biotech research. 2bind was able to quickly and efficiently characterize the affinity of a novel molecule to its target delivering a comprehensible report on short notice which helped us meet our objective.

Huram Konjen, CEO Dianox Dianox
Huram Konjen, CEO Dianox

We used the services of 2bind several times, including MST, BLI and nanoDSF and were always happy with the excellent service and result quality.

Philipp Richle CDR-Life AG
Philipp Richle

We are impressed with the rapid turnaround time and excellent service, as well the high quality data generated at 2bind.

Brian Hamman, Director of Biophysics Arvinas, Inc.
Brian Hamman, Director of Biophysics

The [2bind] assay development was well-planned, careful and methodical. Progress reports were frequent, very clear and meticulously prepared. Their science and professionalism are impeccable – working with 2bind is like having expert biophysicists in the lab next door!

Chris Lepre, PhD, Biochemistry, Biophysics and Structural Biology Relay Therapeutics, Inc.
Chris Lepre, PhD, Biochemistry, Biophysics and Structural Biology

The MST experts at 2bind were successfull in setting up various binding assays, stoichiometry assays, and competition assays and helped us to drive our science. We are very satisfied with the low sample consumption and the fast turnaround time.

Mads Nøregaard-Madsen, Chief Innovation & Information Officer Nuevolution
Mads Nøregaard-Madsen, Chief Innovation & Information Officer

During several years we have had excellent experience working with 2bind. They have always provided us with the highest-quality services. Quick responsiveness of 2bind’s team has helped us to accelerate projects.

Dmitry Shishov Gero
Dmitry Shishov
Assay development, quantitative biology, high-throughput screening, in vitro biactivity, lab automation.
NanoTemper-certified service provider for MST and nanoDSF. Collaboration in beta-testing or instruments and consumables.
AI-based chemical library screening, protein off-target prediction, chemical hit diversification.
Integrated DRIVE™ drug discovery and development platform.
Lead compound selection, mass spectrometry-based on- and off-target analysis and target deconvolution.
GMP-compliant production of recombinant proteins, cell line development.
swithSENSE technology, cooperation for assay and method development.

Structure-guided hit validation with proprietary crystallization platform.

Find out more about our partners and the 2bind Analytics Service Network here!

Media and Ressources

We are sure there is a service for your exact needs!
Have a look at the 2bind Application Notes and peer-reviewed publications to see what we can do!

Contact Specialist