Work with the Experts in Biophysics
Our mission at 2bind is to accelerate your research by developing and performing robust, high-end, scalable, and cost-efficient biophysical assays for:
Drug Discovery
Fragment-based Drug Discovery
Protein Interactions and Stability
Antibody Development
Aptamer Research
RNA-targeted Drug Discovery
Services for Drug Discovery
- High-throughput screening assay development
- In-house compound libraries (5 000 and 150 000 library sizes)
- High-throughput compound handling (LabCyte Echo platform)
- High-throughput biophysical screening (MST and nanoDSF)
- Hit-Validation (MST, nanoDSF, BLI, and ITC based hit confirmation assays)
- Hit-Characterization (in-depth analysis of lead affinity, kinetics, thermodynamics, stoichiometry, influence of bioliquids)
- In silico pre-screening of up to 3 Million compounds
Services for Fragment-based Drug Discovery
- High-throughput screening assay development
- In-house high-diversity fragment library (2 000 fragments library sizes)
- High-throughput fragment handling (LabCyte Echo platform)
- High-throughput biophysical screening (MST and nanoDSF)
- Hit-Validation (MST, nanoDSF, BLI, and ITC based hit confirmation assays)
Services for Protein Interaction Analysis
- Steady-state binding assay (affinity, KD), MST, nanoDSF, BLI, ITC
- High-throughput binding screening (MST, nanoDSF)
- Kinetic binding assays (BLI)
- Thermodynamic profiling (ITC; binding enthalpy and entropy, free enthalpy of binding, rection stoichiometry)
- Assays in bioliquids (bio-availability, off-target effects)
- Competition assays (ligand displacement, complex formation)
- Thermal-shift binding assays
Services for Protein Stability Analysis
- Buffer screening and optimization (nanoDSF; custom or pre-made buffers, 96-well buffer plates available)
- Long-term storage optimization (quality control)
- Protein batch comparison
- Forced-degradation assays
Services for Antibody-Target Interactions
- Steady-state binding assay (affinity, KD), MST, BLI, ITC
- Kinetic binding assays (BLI)
- Thermodynamic profiling (ITC; binding enthalpy and entropy, free enthalpy of binding, rection stoichiometry)
- Assays in bioliquids (bio-availability, off-target effects)
- Epitope binning (discrimination between different epitopes on one antigen)
- Antibody quantitation in culture supernatants
Services for Antibody Stability and Aggregation
- Buffer screening and optimization (nanoDSF; custom or pre-made buffers, 96-well buffer plates available)
- Antibody pre-formulation development (stability analysis of antibodies in 96 buffer conditions from FDA- and EMA-approved therapeutic antibodies)
- Long-term storage optimization (quality control)
- Biosimilar comparison
- Forced-degradation assays
- Deep-feature, mutation, and glycosylation analysis
Services for Aptamer Research
- Steady-state binding assay (affinity, KD, MST, BLI, ITC)
- Kinetic binding assays (BLI)
- High-throughput screening assays (ligand binding screen, MST)
- Thermodynamic profiling (ITC; binding enthalpy and entropy, free enthalpy of binding, rection stoichiometry)
- Assays in bioliquids (bio-availability, off-target effects)
- Sandwich assays (Interaction site mapping)
- Competition assays
Services for RNA-targeted Drug Discovery
- Steady-state binding assay (affinity, KD, MST, BLI, ITC)
- High-throughput screening assays (ligand binding screen, MST)
- Kinetic binding assays (BLI)
- Thermodynamic profiling (ITC; binding enthalpy and entropy, free enthalpy of binding, rection stoichiometry)
- Assays in bioliquids (bio-availability, off-target effects)
- Competition assays
Our Mindset - Working with us
2bind is one of the world’s leading service providers for biophysics-based assays. We have worked with 6 of the top 10 pharmaceutical companies world-wide – e.g. Roche – and with many top-tier Universities – e.g. MIT and Stanford. Just as well, many SMEs and life-science start-ups trust us with their biophysical assay projects.
Milestone-based projects
Milestones and exit-options give you the full control over your projects.
Clear cost-structure
All costs are known prior to project start. Cancelled milestones are not charged.
Fast turn-around times
The structured project pipeline ensures turn-around times of few working days even for complex screening projects.
Long-standing expertise
2bind is the first CRO certified by NanoTemper for MST and nanoDSF.
Personal project manager
All customer projects are handled by highly skilled, PhD-level biochemists and biophysicists.
Customers from Pharma, Biotech, and Academia
Completed contract research projects
Years of experience with MST and nanoDSF
Protein, DNA, RNA targets analyzed
State-of-the-art Biophysics
Our state-of-the-art portfolio of biophysical technologies enables us to provide you with a complete and comprehensive picture of your specific question: From affinity and stability to kinetics and thermodynamics.
KD | EC50 | Tm | ΔTm | Tagg | ΔTagg | kon | koff | n | ΔH | ΔG | ΔS
MST
- Steady-state affinity
- Ligand binding
- Screening
- Proteins, DNA, RNA
- Bioliquids
nanoDSF
- Protein stability
- Protein unfolding
- Protein aggregation
- Ligand binding
- Screening
- Bioliquids
BLI
- Kinetics
- Ligand binding
- Antibody development
- Off-rate ranking
- Bioliquids
ITC
- Thermodynamics
- Stoichiometry
- Ligand binding
Clients & Partners
We have been using NanoTemper devices for over eight years and have established a close and very productive relationship together. 2bind has the highest density of NanoTemper devices in one lab world-wide.
Assay.Works is our go-to partner for all biochemical and cell-based assay development and screening requirements. We partner also in the area of ligand compound and bioactive tool compound libraries.