Work with the Experts in Biophysics

New: The heliX Biosensor

Unrivaled tool for demanding bioanalysis.

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New: Creoptix WAVEsystem

The new gold-standard in rapid kinetic analysis: Grating-coupled interferometry.

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Our mission at 2bind is to accelerate your research by developing and performing robust, high-end, scalable, and cost-efficient biophysical assays for:

Drug Discovery

Fragment-based Drug Discovery

RNA-targeted Drug Discovery

Protein Interactions and Stability

Antibody Development

Aptamer Research

Services for Drug Discovery

  • High-throughput screening assay development
  • In-house compound libraries (5 000 and 150 000 library sizes)
  • High-throughput compound handling (LabCyte Echo platform)
  • High-throughput biophysical screening (MST, Spectral Shift, GCI, Dianthus, nanoDSF)
  • Hit-Validation (MST, Spectral Shift, GCI, Dianthus, nanoDSF, BLI, and ITC based hit confirmation assays)
  • Hit-Characterization (in-depth analysis of lead affinity, kinetics, thermodynamics, stoichiometry, influence of bioliquids)
  • In silico pre-screening of up to 3 Million compounds
  • Integrated drug discovery platform (from hit ID to IND filing)
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Services for Fragment-based Drug Discovery

  • High-throughput screening assay development
  • In-house fragment libraries (2 000 and 3 600 library sizes)
  • High-throughput fragment handling (LabCyte Echo platform)
  • High-throughput biophysical screening (MST, Spectral Shift, GCI, Dianthus, nanoDSF)
  • Hit-Validation (MST, Spectral Shift, GCI, Dianthus, nanoDSF, and ITC based hit confirmation assays)
  • In silico pre-screening of fragments
  • Integrated drug discovery program (from fragment ID to IND filing)
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Services for RNA-targeted Drug Discovery

  • High-throughput screening assay development
  • In-house compound libraries (5 000 and 150 000 library sizes)
  • In-house fragment libraries (~ 2000 library size)
  • High-throughput compound handling (LabCyte Echo platform)
  • High-throughput biophysical screening (MST, Spectral Shift, Dianthus, GCI)
  • Hit-Validation (MST, Spectral Shift, GCI, BLI, and ITC based hit confirmation assays)
  • Hit-Characterization (in-depth analysis of lead affinity, kinetics, thermodynamics, stoichiometry, influence of bioliquids)
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Services for Protein Interaction Analysis

  • Steady-state binding assay (affinity, KD), MST, nanoDSF, BLI, ITC
  • High-throughput binding screening (MST, nanoDSF)
  • Kinetic binding assays (BLI)
  • Thermodynamic profiling (ITC; binding enthalpy and entropy, free enthalpy of binding, rection stoichiometry)
  • Assays in bioliquids (bio-availability, off-target effects)
  • Competition assays (ligand displacement, complex formation)
  • Thermal-shift binding assays
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Services for Protein Stability Analysis

  • Buffer screening and optimization (nanoDSF; custom or pre-made buffers, 96-well buffer plates available)
  • Long-term storage optimization (quality control)
  • Protein batch comparison
  • Forced-degradation assays
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Colloidal, Thermal, Chemical Stability

  • High-throughput developability screens
  • Buffer compatibility screens
  • In-depth characterization
    • Long-term storage stability
    • Stress tests
    • Antibody pre-formulation
    • Forced-degradation assays
    • QC
    • Batch release
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Affinity, Avidity, Quantification

  • High-throughput affinity/avidity screens
  • Early-stage clone ranking (kon, koff, KD)
  • Antibody quantitation in cell culture supernatants
  • Avidity screens of immune sera
  • In-depth characterization of binding affinity and avidity
    • Epitope binning
    • Off-target screens
    • Real-time kinetics on target cells
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Services for Aptamer Research

  • Steady-state binding assay (affinity, KD, MST, Spectral Shift, Dianthus, GCI, ITC)
  • Kinetic binding assays (GCI, heliX, BLI)
  • High-throughput screening assays (ligand binding screen, MST, Spectral Shift, GCI)
  • Thermodynamic profiling (ITC; binding enthalpy and entropy, free enthalpy of binding, rection stoichiometry)
  • Assays in bioliquids (bio-availability, off-target effects)
  • Sandwich assays (Interaction site mapping)
  • Competition assays
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Our Mindset - Working with us

2bind is one of the world’s leading service providers for biophysics-based assays. We have worked with 6 of the top 10 pharmaceutical companies world-wide – e.g. Roche – and with many top-tier Universities – e.g. MIT and Stanford. Just as well, many SMEs and life-science start-ups trust us with their biophysical assay projects.

Milestone-based projects

Milestones and exit-options give you the full control over your projects.

Clear cost-structure

All costs are known prior to project start. Cancelled milestones are not charged.

Fast turn-around times

The structured project pipeline ensures turn-around times of few working days even for complex screening projects.

Long-standing expertise

2bind is the first CRO certified by NanoTemper for MST and nanoDSF.

Personal project manager

All customer projects are handled by highly skilled, PhD-level biochemists and biophysicists.

Customers from Pharma, Biotech, and Academia

Completed contract research projects

Years of experience with MST and nanoDSF

Protein, DNA, RNA targets analyzed

State-of-the-art Biophysics

Our cutting-edge portfolio of biophysical technologies provides a comprehensive answer to your question:
From binding to affinity.
From stability and folding to formulation.
From kinetics to thermodynamics.

KD | EC50 | Tm | ΔTm | Tagg | ΔTagg | kon | koff | n | ΔH | ΔG | ΔS | rh | PDI | Tsize | kD

Automated MST

APPLICATION AREAS

  • Drug discovery
  • Fragment screening
  • Compound screening
  • Protein interaction analysis

PARAMETERS

  • Single-dose binding
  • Steady-state affinity (KD)

NANOTEMPER CERTIFIED SERVICE PROVIDER

Dianthus

APPLICATION AREAS

  • Drug discovery
  • Fragment screening
  • Compound screening

PARAMETERS

  • Single-dose binding
  • Steady-state affinity (KD)

NANOTEMPER CERTIFIED SERVICE PROVIDER

WAVEsystem

APPLICATION AREAS

  • Drug discovery
  • Fragment screening
  • Compound screening
  • Off-rate ranking
  • Biologics development

PARAMETERS

  • Binding kinetics (kon, ka, koff, kd)
  • Steady-state affinity (KD)
  • Enthalpy (ΔH)

CREOPTIX CERTIFIED SERVICE PROVIDER

MicroScale Thermophoresis (MST Service) is a biophysical technique that measures the strength of interaction (affinity) between two molecules by detecting variations in fluorescence signal as a result of an IR-laser induced temperature change. The range of the variation in the fluorescence signal correlates with the binding of a ligand to the fluorescent target. By that it allows for quantitative analysis of molecular interactions in solution on the microliter scale with high sensitivity. This page gives an overview of the MicroScale Thermophoresis (MST) method. You can find information on the core technology and typical applications of MST. Also detailed information on labeling and fluorescence requirements and the central advantages of the method are given.

MST

APPLICATION AREAS

  • Small-scale interaction analysis
  • Assay development
  • Aptamer binding
  • Protein-protein interaction analysis
  • Competition assays

PARAMETERS

  • Single-dose binding
  • Steady-state affinity (KD)

NANOTEMPER CERTIFIED SERVICE PROVIDER

Helix biosensor - SwitchSense - Dynamic biosensors for measuring binding kinetics, conformational change, bi-specific antibodies, PROTACs, molecular glue, enzyme kinetics, single cell binding kinetics - Bioanalytics with Biophysics at 2bind

heliX biosensor

APPLICATION AREAS

  • Broad-range kinetic binding
  • Molecular conformation
  • Multi-specific binders
  • PROTACs
  • Nucleic acids targeting
  • Enzymatic activity

PARAMETERS

  • Binding kinetics (kon, ka, koff, kd)
  • Steady-state affinity (KD)
  • Conformation
  • Ternary complex formation

DBS-CERTIFIED SERVICE PROVIDER

BLI

APPLICATION AREAS

  • Biologics development
  • Antibody-antigen interaction analysis
  • Off-rate ranking

PARAMETERS

  • Binding kinetics (kon, ka, koff, kd)
  • Steady-state affinity (KD)
  • Enthalpy (ΔH)

nanoDSF

APPLICATION AREAS

  • Protein stability and unfolding analysis
  • Buffer screening and optimization
  • Protein aggregation analysis
  • Pre- and formulation development
  • Ligand binding (thermal shift)

PARAMETERS

  • Protein unfolding (Tm, Tonset, Tturbidity, kd)
  • Binding (Tm-diff, EC50)

NANOTEMPER CERTIFIED SERVICE PROVIDER

DLS - Panta

APPLICATION AREAS

  • Protein quality control
  • Particle size determination
  • Solutio homogeneity
  • Protein aggregation analysis
  • Pre- and formulation development
  • Buffer screening and optimization

PARAMETERS

  • Paticle parameters (rh, PDI)
  • Aggregation analysis (Tsize, Tscattering)

NANOTEMPER CERTIFIED SERVICE PROVIDER

ITC

APPLICATION AREAS

  • Thermodynamic interaction analysis
  • Drug discovery
  • Compound and fragment binding
  • Protein-protein interaction analysis

PARAMETERS

  • Steady-state affinity (KD)
  • Binding stoichiometry (n)
  • Binding thermodynamics (ΔH, ΔS, ΔG)

DLS - Wyatt

APPLICATION AREAS

  • Protein quality control
  • Particle size determination
  • Compound solubility
  • Solutio homogeneity
  • Protein aggregation analysis
  • Pre- and formulation development
  • Buffer screening and optimization
  • DoE setups

PARAMETERS

  • Paticle parameters (rh, PDI, MW)
  • Aggregation analysis (Tsize, Tscattering)

WYATT-CERTIFIED SERVICE PROVIDER

LabCyte Echo

APPLICATION AREAS

  • Compound spotting/printing
  • Fragment spotting/printing
  • Contact-free, high-precision dilution series
  • Industry-standard

Clients & Partners

Working with 2bind was a great experience. As a deep tech company, collaborations with specialists are important to navigate complex biotech research. 2bind was able to quickly and efficiently characterize the affinity of a novel molecule to its target delivering a comprehensible report on short notice which helped us meet our objective.

Huram Konjen, CEO Dianox Dianox
Huram Konjen, CEO Dianox

We used the services of 2bind several times, including MST, BLI and nanoDSF and were always happy with the excellent service and result quality.

Philipp Richle CDR-Life AG
Philipp Richle

We are impressed with the rapid turnaround time and excellent service, as well the high quality data generated at 2bind.

Brian Hamman, Director of Biophysics Arvinas, Inc.
Brian Hamman, Director of Biophysics

The [2bind] assay development was well-planned, careful and methodical. Progress reports were frequent, very clear and meticulously prepared. Their science and professionalism are impeccable – working with 2bind is like having expert biophysicists in the lab next door!

Chris Lepre, PhD, Biochemistry, Biophysics and Structural Biology Relay Therapeutics, Inc.
Chris Lepre, PhD, Biochemistry, Biophysics and Structural Biology

The MST experts at 2bind were successfull in setting up various binding assays, stoichiometry assays, and competition assays and helped us to drive our science. We are very satisfied with the low sample consumption and the fast turnaround time.

Mads Nøregaard-Madsen, Chief Innovation & Information Officer Nuevolution
Mads Nøregaard-Madsen, Chief Innovation & Information Officer

During several years we have had excellent experience working with 2bind. They have always provided us with the highest-quality services. Quick responsiveness of 2bind’s team has helped us to accelerate projects.
‌

Dmitry Shishov Gero
Dmitry Shishov

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Assay development, quantitative biology, high-throughput screening, in vitro biactivity, lab automation.
NanoTemper-certified service provider for MST and nanoDSF. Collaboration in beta-testing or instruments and consumables.

Creoptix-certified service provider for GCI/WAVE kinetic analysis. Collaboration in beta-testing or instruments and consumables.

AI-based chemical library screening, protein off-target prediction, chemical hit diversification.
Integrated DRIVE™ drug discovery and development platform.
Lead compound selection, mass spectrometry-based on- and off-target analysis and target deconvolution.
GMP-compliant production of recombinant proteins, cell line development.

heliX® biosensor and switchSENSE® technology, cooperation for assay and method development.

Structure-guided hit validation with proprietary crystallization platform.

Find out more about our partners and the 2bind Analytics Service Network here!

Media and Ressources

We are sure there is a service for your exact needs!
Have a look at the 2bind Application Notes and peer-reviewed publications to see what we can do!

Application Notes

Publications

Press and Business Releases

Contact Specialist