Work with the Experts in Biophysics

Check out our new service for
FRAGMENT-BASED DRUG DISCOVERY
and our new product line:
FDA-plate

Our mission at 2bind is to accelerate your research by developing and performing robust, high-end, scalable, and cost-efficient biophysical assays for:

Drug Discovery

Fragment-based Drug Discovery

Protein Interactions and Stability

Antibody Development

Aptamer Research

RNA-targeted Drug Discovery

Services for Drug Discovery

  • High-throughput screening assay development
  • In-house compound libraries (5 000 and 150 000 library sizes)
  • High-throughput compound handling (LabCyte Echo platform)
  • High-throughput biophysical screening (MST and nanoDSF)
  • Hit-Validation (MST, nanoDSF, BLI, and ITC based hit confirmation assays)
  • Hit-Characterization (in-depth analysis of lead affinity, kinetics, thermodynamics, stoichiometry, influence of bioliquids)
  • In silico pre-screening of up to 3 Million compounds

Services for Fragment-based Drug Discovery

  • High-throughput screening assay development
  • In-house high-diversity fragment library (2 000 fragments library sizes)
  • High-throughput fragment handling (LabCyte Echo platform)
  • High-throughput biophysical screening (MST and nanoDSF)
  • Hit-Validation (MST, nanoDSF, BLI, and ITC based hit confirmation assays)

Services for Protein Interaction Analysis

  • Steady-state binding assay (affinity, KD), MST, nanoDSF, BLI, ITC
  • High-throughput binding screening (MST, nanoDSF)
  • Kinetic binding assays (BLI)
  • Thermodynamic profiling (ITC; binding enthalpy and entropy, free enthalpy of binding, rection stoichiometry)
  • Assays in bioliquids (bio-availability, off-target effects)
  • Competition assays (ligand displacement, complex formation)
  • Thermal-shift binding assays

Services for Protein Stability Analysis

  • Buffer screening and optimization (nanoDSF; custom or pre-made buffers, 96-well buffer plates available)
  • Long-term storage optimization (quality control)
  • Protein batch comparison
  • Forced-degradation assays

Services for Antibody-Target Interactions

  • Steady-state binding assay (affinity, KD), MST, BLI, ITC
  • Kinetic binding assays (BLI)
  • Thermodynamic profiling (ITC; binding enthalpy and entropy, free enthalpy of binding, rection stoichiometry)
  • Assays in bioliquids (bio-availability, off-target effects)
  • Epitope binning (discrimination between different epitopes on one antigen)
  • Antibody quantitation in culture supernatants

Services for Antibody Stability and Aggregation

  • Buffer screening and optimization (nanoDSF; custom or pre-made buffers, 96-well buffer plates available)
  • Antibody pre-formulation development (stability analysis of antibodies in 96 buffer conditions from FDA- and EMA-approved therapeutic antibodies)
  • Long-term storage optimization (quality control)
  • Biosimilar comparison
  • Forced-degradation assays
  • Deep-feature, mutation, and glycosylation analysis

Services for Aptamer Research

  • Steady-state binding assay (affinity, KD, MST, BLI, ITC)
  • Kinetic binding assays (BLI)
  • High-throughput screening assays (ligand binding screen, MST)
  • Thermodynamic profiling (ITC; binding enthalpy and entropy, free enthalpy of binding, rection stoichiometry)
  • Assays in bioliquids (bio-availability, off-target effects)
  • Sandwich assays (Interaction site mapping)
  • Competition assays 

Services for RNA-targeted Drug Discovery

  • Steady-state binding assay (affinity, KD, MST, BLI, ITC)
  • High-throughput screening assays (ligand binding screen, MST)
  • Kinetic binding assays (BLI)
  • Thermodynamic profiling (ITC; binding enthalpy and entropy, free enthalpy of binding, rection stoichiometry)
  • Assays in bioliquids (bio-availability, off-target effects)
  • Competition assays

Our Mindest - Working with us

2bind is one of the world’s leading service providers for biophysics-based assays. We have worked with 6 of the top 10 pharmaceutical companies world-wide – e.g. Roche – and with many top-tier Universities – e.g. MIT and Stanford. Just as well, many SMEs and life-science start-ups trust us with their biophysical assay projects.

Milestone-based projects

Milestones and exit-options give you the full control over your projects.

Clear cost-structure

All costs are known prior to project start. Cancelled milestones are not charged.

Fast turn-around times

The structured project pipeline ensures turn-around times of few working days even for complex screening projects.

Long-standing expertise

2bind is the first CRO certified by NanoTemper for MST and nanoDSF.

Personal project manager

All customer projects are handled by highly skilled, PhD-level biochemists and biophysicists.

Customers from Pharma, Biotech, and Academia

Completed contract research projects

Years of experience with MST and nanoDSF

Protein, DNA, RNA targets analyzed

State-of-the-art Biophysics

Our state-of-the-art portfolio of biophysical technologies enables us to provide you with a complete and comprehensive picture of your specific question: From affinity and stability to kinetics and thermodynamics.

KD | EC50 | Tm | ΔTm | Tagg | ΔTagg | kon | koff | n | ΔH | ΔG | ΔS

MST

  • Steady-state affinity
  • Ligand binding
  • Screening
  • Proteins, DNA, RNA
  • Bioliquids

nanoDSF

  • Protein stability
  • Protein unfolding
  • Protein aggregation
  • Ligand binding
  • Screening
  • Bioliquids

BLI

  • Kinetics
  • Ligand binding
  • Antibody development
  • Off-rate ranking
  • Bioliquids

ITC

  • Thermodynamics
  • Stoichiometry
  • Ligand binding

Clients & Partners

We used the services of 2bind several times, including MST, BLI and nanoDSF and were always happy with the excellent service and result quality.

Philipp Richle  CDR-Life AG
Philipp Richle

We are impressed with the rapid turnaround time and excellent service, as well the high quality data generated at 2bind.

Brian Hamman, Director of Biophysics  Arvinas, Inc.
Brian Hamman, Director of Biophysics

The [2bind] assay development was well-planned, careful and methodical. Progress reports were frequent, very clear and meticulously prepared. Their science and professionalism are impeccable – working with 2bind is like having expert biophysicists in the lab next door!

Chris Lepre, PhD, Biochemistry, Biophysics and Structural Biology  Relay Therapeutics, Inc.
Chris Lepre, PhD, Biochemistry, Biophysics and Structural Biology

The MST experts at 2bind were successfull in setting up various binding assays, stoichiometry assays, and competition assays and helped us to drive our science. We are very satisfied with the low sample consumption and the fast turnaround time.

Mads Nøregaard-Madsen, Chief Innovation & Information Officer  Nuevolution
Mads Nøregaard-Madsen, Chief Innovation & Information Officer

During several years we have had excellent experience working with 2bind. They have always provided us with the highest-quality services. Quick responsiveness of 2bind’s team has helped us to accelerate projects.

Dmitry Shishov  Gero
Dmitry Shishov

We have enjoyed the quality of service of 2bind coupled to a great flexibility. 2bind’s technology allowed us to quickly assess target validation with dozens of proteins and helped us advance quickly on the subject.

Philippe Verwaerde, President and CSO  AlzProtect
Philippe Verwaerde, President and CSO

We have been using NanoTemper devices for over eight years and have established a close and very productive relationship together. 2bind has the highest density of NanoTemper devices in one lab world-wide.

Assay.Works is our go-to partner for all biochemical and cell-based assay development and screening requirements. We partner also in the area of ligand compound and bioactive tool compound libraries.

Media and Ressources

We are sure there is a service for your exact needs!
Have a look at the 2bind Application Notes and peer-reviewed publications to see what we can do!

Contact Specialist