We’ve worked closely with 2bind to develop MST and nano-DSF assays for two projects. The assay development was well-planned, careful and methodical. Unlike some CROs that need to be coached, the highly experienced 2bind team worked independently and found creative solutions to run assays on several unstable and poorly behaved proteins. Progress reports were frequent, very clear and meticulously prepared. We eventually scaled up to run a weekly MST assay to support ongoing chemistry, with hundreds of compounds tested and results consistently returned in less than a week (even though we shipped compounds from the US to Germany). Their science and professionalism are impeccable – working with 2bind is like having expert biophysicists in the lab next door.
Our company is constantly looking for innovations and improvements in drug discovery processes. MicroScale Thermophoresis represents a novel method, which has proven well suited for the characterization of protein-small compound interactions in solution. We have worked with 2bind on several projects and we are very satisfied with the low sample consumption and the fast turnaround time. The MST experts at 2bind were successfully setting up various binding assays, stoichiometry assays, and competition assays and helped us to drive our science. We are looking forward to a continued collaboration.
Gero is a biotech company focused on drug development. During several years we have had excellent experience working with 2bind on different R&D projects including small molecules, oligonucleotides and antibodies. 2bind has always provided us with the highest-quality services using modern sophisticated methods of binding studies with high reliability of the results. It is worth noting that these results can be used as a reference in case of inconclusive data from other labs. I am completely satisfied with our cooperation and Thomas has always helped us to understand features and pitfalls of the experiments. Quick responsiveness of 2bind’s team has helped us to accelerate projects.
We have been working with 2bind for two years now with great success. We have enjoyed the quality of service coupled to a great flexibility. Results always come with a pertinent scientific advice. 2bind’s technology allowed us to quickly assess target validation with dozens of proteins and helped us advance quickly on the subject.
Lysando AG is a technological leader in the field of antimicrobial proteins, which are used to combat multidrug resistant bacteria. Central goals of our protein design and formulation are the correct folding and a high stability of these proteins. Next to CD spectroscopy, we use nanoDSF analyses performed by the experienced team of 2bind in order to gain insights into the desired protein parameters. Beside the fast realization and the low sample consumption, we are impressed by the great consistency of both methods. The highly reproducible nanoDSF data provided important information on the stability of our proteins under diverse buffer conditions.
We have greatly enjoyed the expert services of 2bind as part of our validation in our custom aptamer development services. The low material consumption and all-solution-phase measurement offered by MST technology represents a great advantage over most of the other options for binding studies. Additionally, the expert staff scientists at 2bind have been willing to put in extra time and effort to optimize our aptamer studies as well as develop certain non-standard protocols specific for our studies. We look forward to continuing to work with 2bind.
I was very impressed with the 2bind team in terms of their problem solving expertise and willingness to go the extra mile for us.
The excellent service offered by 2bind allowed us to identify novel protein-nucleic acid interactions, that in the following could be validated convincingly by other experimental approaches. We enjoyed the pleasant and successful collaboration with 2bind.
With the help of 2bind and their superb analytical capabilities we were able to gain fundamental scientific insights. I am really satisfied with their customer support, speed, and quality of results and would definitely recommend their services.
Our research group at the Helmholtz Centre for environmental research (UFZ) is focusing on the development of aptamers for applications in environmental and food analytics. Aptamers serve as recognition elements in biosensors and assays to detect environmental pollutants. Our group possesses a longstanding expertise in the selection of aptamers mainly to small molecules and proteins. We characterize our aptamers in terms of binding affinity and selectivity using different state of the art technologies. The MST experts at 2bind supported us with MicroScale Thermophoresis analyses, which were performed in a fast and efficient manner. We were very satisfied with the high quality of the MST data, which nicely correlated with other biophysical approaches we routinely perform.
One of the main topics in our lab is the characterization of Protein-RNA interactions. As the MST technology is optimal for the analysis of these interactions, we decided to work closely with the MST experts at 2bind. We were very pleased with the fast progress of the projects and absolutely satisfied with the final data. The broad knowledge of the staff at 2bind and their excellent expertise in the MST technology, were the basis of our successful collaboration. We would highly recommend 2bind to other colleagues.
We routinely use a variety of techniques to evaluate the interactions between aptamers and their cognitive targets and recently had an extremely positive experience of the services offered by 2bind for the microscale thermophoretic analysis of binding affinities. Not only was the amount of material required for the analysis extremely low, 2bind offered very valuable advice in assay design and definitely went the extra mile in elucidating optimal interaction conditions, with high quality, reliable data being produced in an impressive turnaround time. The customer support was excellent, and we will definitely be using their service in the future, and it is a service I can highly recommend, not only to those working in the aptamer field, but in any area involving molecular interactions.