Our Services for Antibody Development
Antibodies in their numerous forms are the backbone of modern therapeutics that target cancer, autoimmune, and infectious diseases. Moreover, highly-specialized antibodies and their derivatives are the basis of precise diagnostic tools.
Antibody development benefits from our broad range of biophysical services in many cases. For example, our tailor-made nanoDSF assays allow for the characterization of antibody stability and aggregation tendency. Moreover, we provide several biophysical assays that allow for the characterization of interactions between antibodies and their targets as well as excluding unwanted, unspecific side-interactions in biological liquids.
Studying Antibody Stability and Antibody Aggregation
Knowing the stability of an antibody is essential in many cases. Our precise, versatile, fast, and cost-efficient nanoDSF platform allows for the determination of antibody stability in a wide range of applications that are outlined below.
For specific examples where antibody stability has been an important part of antibody development, have a look at our application notes. You can also use the contact form below to ask us anything about the services outlined below. We will get back to you shortly with a solution made specifically for your request!
Find the optimal buffer or buffer additives for your antibody in a pre-formulation phase buffer screening. We hold ready up to 500 buffers and a subset of 70 FDA-approved therapeutic antibody formulations.
Long-term Storage Optimization
Identify the optimal conditions for storing your antibody over a defined period of time. Long-term storage assays can be added as a subsequent module after buffer screening assays.
Forced Degradation Assays
Test the stability of your antibody under different stress conditions. For example, chemically induced unfolding, freeze-thaw cycling, thermal stress, as well as mechanical stress.
Compare your biosimilar antibody to the originator. Comparison assays are modular and can combine buffer screenings, long-term storage tests, as well as forced degradation analysis.
Deep Feature Analysis
Determine the influence of amino-acid substitutions, chemical modifications like drug- or dye-conjugates, or other types of modifications on the stability of your antibody.
Characterizing Antibody Target Interactions
Precise information about the interaction of an antibody either with its natural antigen or with a variety of other types of targets is key for developing therapeutic and diagnostic antibodies. Our versatile toolbox of biophysical methods is tailored towards maximum flexibility in investigating several aspects of antibody target interactions.
If you want to read more on the biophysical and technical background of the applied methods, please have a look at our technology section. For examples of antibody target interactions, we would like to refer you to our application notes. You can also use the contact form below to ask us anything about the services outlined below. We will get back to you shortly with a solution made specifically for your request!
Steady-state Binding Assay - MST, BLI, ITC
Determine the steady-state affinity (KD) of your antibody to its natural antigen or to any possible target: ranging from ions and small molecules, over peptides, DNA, and RNA, to proteins or synthetic particles.
Dynamic Kinetic Assay - BLI
Study on- and off-rates (kon, koff) of interactions between antibodies and antigens or other targets. Compare different antibodies with respect to their kinetic behavior using our BLI-platform.
Thermodynamics Assay - ITC
Characterize antibody-antigen or antibody-target interactions with respect to their thermodynamics parameters including enthalpy (ΔH), free enthalpy of binding (ΔG), entropy (ΔS), and stoichiometry using ITC.
Assays in Biological Liquids - MST
Determine antibody-target interactions in relevant biological liquids such as serum, plasma, cell lysate, urine, mucose, or environmental matrices. Exclude unwanted side-interactions in these biological matrices.