Services for Antibody Development

Antibodies in their numerous forms are the backbone of modern therapeutics that target cancer, autoimmune, and infectious diseases. Moreover, highly-specialized antibodies and their derivatives are the basis of precise diagnostic tools.

Antibody developers benefit from our broad range of biophysical services. Interactions between antibodies and antigens can be analyzed with a variety of biophysical methods (see below). Antibody stability and aggregation is easily acceesible via our tailor-made nanoDSF assays.

Optimization of antibody buffer and storage conditions as well as development of pre-formulations is now easier than ever with the 2bind FDA-plate(R): A collection of 96 ready-to-use buffer conditions from FDA- and EMA-approved therapeutic antibodies. With the FDA-plate you can use the same antibody storage buffers as the experts do. Get more information here!

Characterizing Antibody Target Interactions

Precise information about antibody-target interactions is key for developing therapeutic and diagnostic antibodies. Our versatile toolbox of biophysical methods (MST, BLI, and ITC) is tailored towards maximum flexibility in investigating several aspects of antibody target interactions.

If you want to read more on the biophysical and technical background of the applied methods, please have a look at our technology section. For examples of antibody target interactions, we would like to refer you to our application notes. You can also use the contact form below to ask us anything about the services outlined below. We will get back to you shortly with a solution made specifically for your request!

Antibody Development, Antibody Stability, Antibody Aggregation, Antibody Target Interactions

Steady-state Binding Assay - MST, BLI, ITC

Determine the steady-state affinity (KD) of your antibody to its natural antigen or to any possible target: ranging from ions and small molecules, over peptides, DNA, and RNA, to proteins or synthetic particles.
Antibody Development, Antibody Stability, Antibody Aggregation, Antibody Target Interactions

Dynamic Kinetic Assay - BLI

Study on- and off-rates (kon, koff) of interactions between antibodies and antigens or other targets. Compare different antibodies with respect to their kinetic behavior using our BLI-platform.
Antibody Development, Antibody Stability, Antibody Aggregation, Antibody Target Interactions

Thermodynamics Assay - ITC

Characterize antibody-antigen or antibody-target interactions with respect to their thermodynamics parameters including enthalpy (ΔH), free enthalpy of binding (ΔG), entropy (ΔS), and stoichiometry using ITC.
Antibody Development, Antibody Stability, Antibody Aggregation, Antibody Target Interactions

Assays in Biological Liquids - MST

Determine antibody-target interactions in relevant biological liquids such as serum, plasma, cell lysate, urine, mucose, or environmental matrices. Exclude unwanted side-interactions in these biological matrices.
Antibody Development, Antibody Stability, Antibody Aggregation, Antibody Target Interactions

Epitope Binning - BLI

Discriminate between different interaction epitopes on the same antigen using in-tandem, competition, or pre-mix kinetic assays.

Antibody Development, Antibody Stability, Antibody Aggregation, Antibody Target Interactions

Antibody Quantification - BLI

Determine the expression level of antibody clones directly in cell culture supernatants with high precision and low detection limits.

Antibody Development, Antibody Stability, Antibody Aggregation, Antibody Target Interactions

High-throughput ligand screening - MST

Analyze your antibody in high-throughput screening assays for binding to up to 1000s of possible antigens, e.g. peptide epitopes.

Studying Antibody Stability and Antibody Aggregation

Knowing the stability of an antibody is essential in many cases. Our precise, versatile, fast, and cost-efficient nanoDSF assays allows for the determination of antibody stability in a wide range of applications outlined below.

For specific examples where antibody stability has been an important part of antibody development, take a look at our application notes. You can also use the contact form below to ask us anything about our antibody services. We will get back to you shortly with a solution made specifically for your request!

Antibody Development, Antibody Stability, Antibody Aggregation, Antibody Target Interactions

Buffer Screening

Find the optimal buffer or buffer additives for your antibody in a pre-formulation phase buffer screening. We can work with specifically designed buffer for your case or with our specialized pre-formulation buffer plate: The FDA-plate.

Antibody Development, Antibody Stability, Antibody Aggregation, Antibody Target Interactions

Long-term Storage Optimization

Identify the optimal conditions for storing your antibody over a defined period of time. Long-term storage assays can be added as a subsequent module after buffer screening assays. Use with our FDA-plate.

Antibody Development, Antibody Stability, Antibody Aggregation, Antibody Target Interactions

Forced Degradation Assays

Test the stability of your antibody under different stress conditions. For example, chemically induced unfolding, freeze-thaw cycling, thermal stress, as well as mechanical stress.
Antibody Development, Antibody Stability, Antibody Aggregation, Antibody Target Interactions

Biosimilar Comparison

Compare your biosimilars to the originator. Comparison assays are modular and can combine buffer screenings, long-term storage tests, as well as forced degradation analysis.

Antibody Development, Antibody Stability, Antibody Aggregation, Antibody Target Interactions

Deep Feature Analysis

Determine the influence of amino-acid substitutions, chemical modifications like drug- or dye-conjugates, or other types of modifications on the stability of your antibody.
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