Services for Protein Biophysics

Protein-protein and protein-ligand interactions are the backbone of almost every vital cellular process and drug discovery project.

We have developed a portfolio of tailor-made services that allow for the precise, robust, fast, and cost-efficient analysis of almost any protein interaction: Proteins and peptides, DNA, RNA, aptamers, small molecules, molecular fragments, ions, viruses, VLPs, and artifical particles. The state-of-the-art biophysical methods MST, nanoDSF, BLI, and ITC are available for in-depth comprehensive interaction analysis, as well as fast and cost-efficient high-throuhput screening.

Moreover, we are able to analyze protein stability, thermal and chemical protein unfolding, protein denaturation and aggregation, as well as buffer optimization, using the state-of-the-art, label-free, in-solution nanoDSF method.

If you are interested in specific example applications, please have a look at our application notes. You can also use the contact form below to ask us anything about the services outlined below. We will get back to you shortly with a solution made specifically for your request!

Services for Protein Interactions

With our tailor-made biophysical assays, interactions between a protein and almost all kinds of relevant ligands can be analyzed: Proteins, peptides, DNA, RNA, aptamers, small molecules, molecular fragments, ions, lipids, virus-like-particles, and artifical particles.

Protein Biophysics, Protein Protein Interactions, Protein Peptide Interactions, Protein Binding, Protein Stability, Protein Denaturation, Protein Aggregation, Protein Unfolding

Steady-state Binding Assay - MST

Determine the steady-state affinity (KD) of your protein or peptide to its natural ligand or to other possible targets, including ions, small molecules, fragments, peptides, DNA, RNA, proteins, or synthetic particles.

Protein Biophysics, Protein Protein Interactions, Protein Peptide Interactions, Protein Binding, Protein Stability, Protein Denaturation, Protein Aggregation, Protein Unfolding

High-throughput screening - MST, nanoDSF

Analyze up to 10 000s of small molecule compounds or molecular fragments for binding to a protein target. Fast, precise, cost-efficient, and with the lowest sample consumption on the market.

Protein Biophysics, Protein Protein Interactions, Protein Peptide Interactions, Protein Binding, Protein Stability, Protein Denaturation, Protein Aggregation, Protein Unfolding

Dynamic Kinetic Assay - BLI

Study on- and off-rates (kon, koff) of interactions between proteins or peptides and their ligands or target. Compare different protein preparations with respect to their kinetic behavior.
Protein Biophysics, Protein Protein Interactions, Protein Peptide Interactions, Protein Binding, Protein Stability, Protein Denaturation, Protein Aggregation, Protein Unfolding

Thermodynamic Profiling - ITC

Characterize protein-target or peptide-target interactions with respect to their thermodynamic parameters, including enthalpy (ΔH), free enthalpy of binding (ΔG), entropy (ΔS), and stoichiometry.
Protein Biophysics, Protein Protein Interactions, Protein Peptide Interactions, Protein Binding, Protein Stability, Protein Denaturation, Protein Aggregation, Protein Unfolding

Assays in Biological Liquids - MST

Determine protein-protein interactions in relevant biological liquids such as serum, plasma, cell lysate, urine, mucose, or environmental matrices. Exclude unwanted side-interactions in these biological matrices.
Protein Biophysics, Protein Protein Interactions, Protein Peptide Interactions, Protein Binding, Protein Stability, Protein Denaturation, Protein Aggregation, Protein Unfolding

Competition Assay - MST, BLI

Determine the ability of your protein or peptide to disrupt the complex of a target molecule and its natural ligand. Alternatively, test the influence of the protein/peptide on interactions between a target and its ligand.

Services for Protein Stability and Unfolding

Information about thermal protein stability is essential in many cases. For example, high protein stability is an important factor for mutational scanning of a protein. Also, therapeutic and diagnostic applications often require proteins to be very stable under the most diverse conditions.

Our precise, versatile, fast, and cost-efficient nanoDSF platform allows for the determination of protein stability in a wide range of applications outlined below. Moreover, nanoDSF allows for the label-free, in-solution analysis of thermal and chemical protein unfolding as well as the detection of protein denaturation and protein aggregation. Importantly, no extrinsic fluorescent dye like in traditional DSF methods is required.

Protein Biophysics, Protein Protein Interactions, Protein Peptide Interactions, Protein Binding, Protein Stability, Protein Denaturation, Protein Aggregation, Protein Unfolding

Protein Stability Buffer Screening

Find the optimal buffer or buffer additives for your protein in a pre-formulation phase buffer screening. We can work with any custom-made buffer you require. Alternatively, the 2bind FDA-plate can be used, offering 96 individual buffers derived from antibody formulations.

Protein Biophysics, Protein Protein Interactions, Protein Peptide Interactions, Protein Binding, Protein Stability, Protein Denaturation, Protein Aggregation, Protein Unfolding

Thermal Shift Binding Assay

Test binding of small molecules, RNA, DNA, peptides, or fragments to proteins by measuring shifts in their thermal stability.

Protein Biophysics, Protein Protein Interactions, Protein Peptide Interactions, Protein Binding, Protein Stability, Protein Denaturation, Protein Aggregation, Protein Unfolding

Long-term Storage Optimization

Identify the optimal conditions for storing your protein over a defined period of time. Long-term storage assays can be added as a subsequent module after buffer screening assays.
Protein Biophysics, Protein Protein Interactions, Protein Peptide Interactions, Protein Binding, Protein Stability, Protein Denaturation, Protein Aggregation, Protein Unfolding

Protein Batch Comparison

Compare different batches of your protein of interest with respect to its thermal stability and thermal unfolding behavior. These assays can be easily combined with buffer screening and long-term stability tests.
Protein Biophysics, Protein Protein Interactions, Protein Peptide Interactions, Protein Binding, Protein Stability, Protein Denaturation, Protein Aggregation, Protein Unfolding

Forced-degradation Assays

Test the stability of your antibody under different stress conditions. For example, chemically induced unfolding, freeze-thaw cycling, thermal stress, as well as mechanical stress.
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