In the recent years, the view of RNA has drastically shifted from the simple messenger between DNA and proteins. Structured and folded RNAs are now recognized as dynamic players in gene expression, pathogenesis, and cancer emergence. Non-coding RNAs are thus more and more in the focus of drug discovery.
We at 2bind are fully aware of this paradigm shift in drug discovery and have thus developed an array of specialized RNA-targeted drug discovery assays outlined below. These assays combine high robustness, comprehensive analysis, and the lowest sample consumption on the market, which is especially important for valuable and expensive RNA molecules.
If you are interested in specific example applications, please have a look at our application notes. You can also use the contact form below to ask us anything about the RNA-targeted drug discovery services outlined below. We will get back to you shortly with a solution made specifically for your request!
Steady-state Binding Assay - MST, BLI, ITC
Determine the steady-state affinity (KD) of your RNA to its natural target or to any other target: ranging from ions and small molecules, over peptides, DNA, and RNA, to proteins or synthetic particles.
High-throughut Screening - MST
Analyze up to 10 000s of small molecule compounds or molecular fragments for binding to an RNA target. Fast, precise, cost-efficient, and with the lowest sample consumption on the market.
Kinetic Binding Assay - BLI
Study on- and off-rates (kon, koff) of interactions between RNAs and target molecules. Compare different aptamers with respect to their kinetic behavior using our BLI-platform.
Thermodynamic Profiling - ITC
Characterize RNA-target interactions with respect to their thermodynamics parameters including enthalpy (ΔH), free enthalpy of binding (ΔG), entropy (ΔS), and stoichiometry using ITC.
Assays in Biological Liquids - MST
Determine RNA-target interactions in relevant biological liquids such as serum, plasma, cell lysate, urine, mucose, or environmental matrices. Exclude unwanted side-interactions in these biological matrices.
Competition Assays - MST, BLI
Determine the ability of your RNA to disrupt the complex of a target molecule and its natural ligand. Alternatively, test the influence of your RNA on interactions between a target and its natural ligand.