In RNA-targeted drug discovery, tackling the complexities of their structures and their dynamic behavior presents significant challenges. At 2bind, our specialized biophysical services are designed to deal with these complex but promising therapeutic targets, providing accurate characterization and empowering informed decision-making.
RNA therapeutics team assembly
RNA target analysis (A priori data analysis, literature research, etc.)
Screening library selection
Deliverable and requirement definition
Selection of nucleic acid-suitable technologies
Material sourcing and outsourcing of RNA synthesis
Reagent qualification
Technical analytical and interaction assay parameter establishment
Positive control establishment for interaction assays
Assay and target robustness testing
Assay screening qualification
True biophysical high-throughput screening
Hight-throughput interaction studies with TRIC (MST), Spectral Shift, GCI, DLS
Library screenings or selected library subsets for focused screening campaigns
Low throughput interaction screens with ITC for smaller subsets
Special screenings of RNA structure changes upon binding with DLS and BLI
Confirmation via in-solution direct binding studies with TRIC (MST) and Spectral Shift
Confirmation via in-solution, label-free direct binding analysis with ITC
Binding confirmation via label-free kinetics GCI assays
Label-free kinetics assay with FPS
Small molecule solubility and aggregation assays with DLS
Target structural changes upon binding with DLS and BLI
Comprehensive in-solution direct binding with TRIC (MST) and Spectral Shift
Comprehensive in-solution, label-free direct binding with ITC
Comprehensive label-free kinetics binding assay with GCI
Comprehensive label-free kinetics binding assay with FPS
Streamlining of suitable hit characterization assays
Regular application of streamlined assays in MedChem optimization cycles
What 2bind delivers for your drug discovery program
