2bind Services – Characterization of Antibodies and Bispecifics

Antibody and bispecifics characterization: 2bind’s biophysical solutions for comprehensive characterization and understanding binary and ternary interactions

Characterization of Antibodies and Bispecifics

Antibodies, especially bispecifics, present a unique challenge: their complex multidomain structures carry multiple potential liabilities. To unlock their full therapeutic potential, we believe in a meticulous, multiparameter characterization approach. This includes testing under both standard and stress conditions to ensure optimal performance.

Avidity effects from bivalency and bispecificity can significantly alter binding behavior compared to simpler 1:1 interactions. In the world of bispecifics, understanding these dual-targeting mechanisms and their intricacies is crucial for success.

At 2bind, our specialized biophysical services are designed precisely for this challenge. We unravel these complexities, providing precise solutions for binding dynamics and specificity, so you can make informed decisions with confidence. Let our expertise guide you towards the successful development of your therapeutics.

What 2bind can do for you
Research and planning
Research and planning

Antibody team assembly

Antibody analysis (A priori data analysis, structural analysis, etc.)

Targets analysis (literature research, mechanistical research, etc.)

Selection of suitable technologies for binary and ternary interactions

Analytical and interaction assay cascade definition

Access and sourcing of material

Binary and ternary interaction assay establishment
Binary and ternary interaction assay establishment

Antibody and bispecifics qualification

Protein targets and reagent qualification

Technical assay parameter establishment for selected technologies

Analytical and interaction assay optimization

Characterization of antibodies and bispecifics
Characterization of antibodies and bispecifics

nanoDSF for conformational stability and high-throughput stability screens

DLS for colloidal stability and self-interaction propensity and high-throughput stability screens

SEC MALS for size determination and fragmentation, oligomerization and aggregation

HIC for hydrophobic profiling

IEX for charge profiling

Binary and ternary interaction studies
Binary and ternary interaction studies

Label-free kinetic binding GCI assays for high-throughput affinity/avidity screens

Label-free kinetic binding BLI assays for affinity/avidity screens in complex matrices, and epitope binning

Kinetics on live cells with Flow Cytometry and RT-IC

In-solution direct binding TRIC (MST), Spectral Shift assays

In-solution, label-free ITC assays for thermodynamic profiling and stoichiometry analysis

Dual color FPS assay for ternary complex analysis and avidity screens

Flow cytometry for high-throughput screens for cell binding

What 2bind delivers for Antibody characterization

In advance

During a project

Afterwards

Get In Touch