2bind Services – PROTACs and Molecular Glues Services

PROTACS and Molecular Glues: 2bind's biophysical Services to enhance your drug discovery program

PROTACs and Molecular Glues Services

In PROTACs and molecular Glues research, understanding the complexities of ternary complex formation is critical. PROTACs recruit the E3 ligase to enforce protein degradation, whereas molecular Glues function by inducing protein-protein interactions with other cellular components. At 2bind, we specialize in providing biophysical services tailored to support PROTACs and molecular Glues discovery efforts. Especially in understanding the ternary complex formation parameters will support your decision-making process.

Screen for binding with TRIC (MST), Spectral Shift, nanoDSF thermal shift assay (TSA), GCI (Grating Coupled Interferometry)
Get binding affinity (K_D) with TRIC (MST), Spectral Shift, ITC (Isothermal Titration Calorimetry), GCI, FPS (Fluorescence Proximity Sensing)
Get binding kinetics (k_on, k_off, K_D) with GCI, FPS
Get molecular analytics parameters of proteins with DLS (Dynamic Light Scattering; r_h, PDI), nanoDSF (Differential Scanning Fluorimetry; T_m, T_agg)
Get molecular analytics parameters of proteins with HPLC (SEC MALS (Size Exclusion Chromatography), HIC (Hydrophobic Interaction Chromatography), IEX (Ion Exchange Chromatography); size, homogeneity, molecular weight, oligomerization state,  hydrophobicity, isoelectric point)
Get molecular analytics parameters of small molecules (solubility, precipitation) with DLS
Get thermodynamic binding information and interaction stoichiometry with ITC
Get overall ternary complex formation data with TRIC (MST), GCI, nanoDSF thermal shift, BLI, dual color FPS, DLS
Get ternary complex formation parameters (K_D, k_on, k_off) for the individual interactions with dual color FPS

What 2bind can do for you

Research and planning

Team assembly

Protein target analysis (literature research, structural analysis, a priori data analysis, etc.)

Deliverable definition

Screening library selection

Enamine High-Fidelity library, 1920 fragments
Enamine Protein-protein interaction fragment library, 3600 fragments
Spirochem sp3-rich Fragments, 480 fragments
Spirochem virtual library, 4.5 Mio compounds
AssayWorks small molecule collection, 100.000 compounds
AssayWorks bioactive collection, 5.050 tool compounds
any other library (sourced externally or brought by the customer)
Spirochem macrocycle collection, 2000 semi-peptidic macrocycles
Any other library (sourced externally or brought by the customer)

Selection of technologies and assay cascade definition

Material access and sourcing

PROTACs and Molecular Glues screening assay establishment

Protein and reagent qualification

Technical assay parameter establishment

Positive control establishment

Screening assay and target robustness testing

Assay screening qualification

Hit identification

True biophysical high-throughput screening

Screenings for binary interaction with TRIC (MST), Spectral Shift, GCI, nanoDSF TSA

Small molecule library binary interaction screening

Selected library subsets for focused screening

Ternary complex formation screenings with TRIC (MST), Spectral Shift, GCI, nanoDSF TSA

Hit confirmation

Binding affinity via TRIC (MST), Spectral Shift, ITC

Binding kinetics via GCI, FPS

Individual binding kinetics for ternary complexes via dual color FPS

Label-free binary and ternary binding analysis via nanoDSF

Hit characterization

Comprehensive in-solution direct binding with TRIC (MST) and Spectral Shift for binary and ternary complexes

Comprehensive in-solution, label-free direct binding with ITC for binary and ternary complexes

In-solution, label-free binding analysis with nanoDSF TSA for binary and ternary complexes

Comprehensive label-free kinetics binding with GCI for binary and ternary complexes

Label-free kinetics binding with BLI for ternary complexes

Label-free kinetics binding with FPS for binary complexes

Dual color FPS dedicated for resolving kinetics of ternary complexes

Thermodynamic characterization with ITC

Ligand structural effect assays with SEC MALS and DLS

Target-aggregator assays with DLS

Hit optimization
Hit to Lead

Streamlining of suitable hit characterization assay

Regular application of streamlined assay in MedChem optimization cycles

SAR support

What 2bind delivers

PROTACS and Molecular Glues: 2bind's biophysical Services to enhance your drug discovery program

PROTACs and Molecular Glues Services

In advance

During a project

Afterwards

Work with the experts in biophysics!