In aptamer discovery, the precision of biophysical tools is essential to understand the specific interactions with their targets. Aptamers are unique due to their structural variability, to be able to bind a wide range of targets and at 2bind, our specialized biophysical services are designed to accurately characterize these interactions. By gaining comprehensive insights into target binding, you can elevate your discovery program.
What 2bind can do for your aptamers
Nucleic acids team assembly
Definition of requirements and deliverables
Selection of nucleic acid-suitable technologies and assay formats
Assay cascade definition
Material sourcing and outsourcing of aptamer synthesis
Reagent qualification
Technical assay parameter establishment
Positive control establishment for the aptamer-target interaction
Assay and target robustness testing
Assay screening qualification
True biophysical high-throughput screening
Hight-throughput interaction studies with TRIC (MST), Spectral Shift, GCI, BLI
Low throughput screening with ITC for smaller subsets
Binding affinity via TRIC (MST), Spectral Shift, ITC
Binding kinetics via GCI, BLI, FPS
Thermodynamic binding analysis via ITC
Aptamer structure change analysis with BLI and DLS
Comprehensive in-solution direct binding with TRIC and Spectral Shift
Comprehensive in-solution, label-free direct binding with ITC
Comprehensive label-free kinetics binding with GC
Comprehensive label-free kinetics binding with BLI
Small molecule solubility and aggregation assays with DLS
Structural effect assays with DLS and BLI
Off-target binding characterization
Streamlining of suitable characterization assays
Regular application of streamlined assay
What 2bind delivers
