Small molecule drug discovery can be initiated from different starting points: High-throughput screenings for a target of interest, in silico screening campaigns, or using an already marketed drug as starting material to improve potency. 2bind’s biophysics services will offer solutions to advance your drug discovery program. Regardless of at which stage your program is, Hit identification to Hit optimization, the 2bind portfolio of biophysics tools, processes and assays will deliver what you need for making your next decisions.
What 2bind can do for your
Small Molecule Drug Discovery
Team assembly
Protein target analysis (literature research, structural analysis, a priori data analysis, etc.)
Screening library selection:
Deliverable definition
Selection of technologies and assay cacade definition
Assay cascade definition
Material access and sourcing
Protein target qualification
Screenung assay technical parameter establishment
Positive control establishment and assay optimization
Assay and protein target robustness testing
Assay screening qualification
True biophysical high-throughput screening
Screening with TRIC (MST), Spectral Shift, GCI, nanoDSF TSA
Library screening
Selected library subsets for focused screening
Competition and allosteric binding screenings
Protein-protein interaction (PPI) displacement screening
Screening of protein-macromolecule complexes (e.g. protein-RNA, protein-DNA)
Binding affinity via TRIC (MST), Spectral Shift, ITC
Binding kinetics via GCI
Label-free binding analysis via nanoDSF TSA
Thermodynamic profiling analysis via ITC
Comprehensive in-solution direct binding with TRIC (MST) and Spectral Shift
Comprehensive in-solution, label-free direct binding with ITC
In-solution, label-free nanoDSF TSA for ligand-induced stability influence
Comprehensive label-free binding kinetics assay with GCI
Comprehensive label-free binding kinetics assay with FPS
Dual color FPS for ternary complex analysis
Ternary complex formation assays with nanoDSF
Competition assays with TRIC (MST), Spectral Shift, nanoDSF, GCI, FPS
Displacement assays with TRIC (MST), Spectral Shift, GCI, FPS
Small molecule solubility and aggregation assays with DLS
Ligand structural effect assays with SEC MALS and DLS
Target-aggregator assays with DLS
Streamlining of suitable hit characterization assay
Regular application of streamlined assay in MedChem optimization cycles
SAR support
What 2bind delivers
