Covalent drug discovery presents unique challenges due to the irreversible nature of covalent bonds, making accurate characterization essential. At 2bind, we specialize in providing biophysical services tailored to support covalent drug discovery efforts. With our expertise in navigating these complexities, we have solutions for analyzing your drugs analytically and to help understanding binding parameters and thermodynamic profiles.
Team assembly
Target analysis (literature research, structural analysis, etc.)
Parameter, deliverable, and assay conditions definition
Screening library selection:
Selection of process specifications (neccessary incubation times, etc.), technologies and assay cascade definition
Material access and sourcing
Protein target and reagent qualification
Technical assay parameter establishment
Positive control establishment and assay optimization
Assay and target robustness testing
Assay screening qualification
True biophysical high-throughput screening
Screening with TRIC (MST), Spectral Shift, GCI, nanoDSF TSA
Library screening
Selected library subsets for focused screening
Competition and allosteric binding screenings
Protein-protein interaction (PPI) displacement screening
Screening of protein-macromolecule complexes (e.g. protein-RNA, protein-DNA)
Binding affinity via TRIC (MST), Spectral Shift, ITC
Binding kinetics via GCI
Label-free binding analysis via nanoDSF TSA
Comprehensive in-solution direct binding with TRIC (MST) and Spectral Shift
Comprehensive in-solution, label-free direct binding with ITC
In-solution, label-free nanoDSF TSA
Comprehensive label-free kinetics binding with GCI
Ligand structural effect assays with SEC MALS and DLS
Target-aggregator assays with DLS
Thermodynamic profiling with ITC
Streamlining of suitable hit characterization assay
Regular application of streamlined assay in fragment evolution MedChem cycles
SAR support
What 2bind delivers
