2bind Services – Fragment-based Drug Discovery Services

Fragment-based Drug Discovery: Use 2bind’s Biophysics Services for competitive timelines and throughput to promote your therapeutic program

Fragment-based Drug Discovery Services

In fragment-based drug discovery, the precision of biophysical tools becomes paramount to deal with extremely small chemical entities. Regardless of your position in the discovery journey, 2bind’s specialized services ensure the delivery of essential insights for informed decision-making. From navigating molecules with weak affinities to handling rapid kinetics, our expertise empowers you to make the right choices at every step.

Screen for binding with TRIC (MST), Spectral Shift, nanoDSF thermal shift assay (TSA), GCI (Grating Coupled Interferometry)
Get binding affinity (K_D) with TRIC (MST), Spectral Shift, ITC (Isothermal Titration Calorimetry), GCI
Get binding kinetics (k_on, k_off, K_D) with GCI
Get molecular analytics parameters of proteins with DLS (Dynamic Light Scattering; r_h, PDI), nanoDSF (Differential Scanning Fluorimetry; T_m, T_agg)
Get molecular analytics parameters of proteins with HPLC (SEC MALS (Size Exclusion Chromatography), HIC (Hydrophobic Interaction Chromatography), IEX (Ion Exchange Chromatography); size, homogeneity, molecular weight, oligomerization state,  hydrophobicity, isoelectric point)
Get molecular analytics parameters of small molecules (solubility, precipitation) with DLS
Get thermodynamic binding information and interaction stoichiometry with ITC

What 2bind can do for you

Research and planning

Team assembly

Protein target analysis (literature research, structural analysis, a priori data analysis, etc.)

Screening library selection:

Enamine High-Fidelity library, 1920 fragments
Enamine Protein-protein interaction fragment library, 3600 fragments
Spirochem sp3-rich Fragments, 480 fragments
Any other library (sourced externally or brought by the customer)

Selection of technologies and assay cascade definition

Material access and sourcing

Assay establishment

Protein target qualification

Screenung assay technical parameter establishment

Positive control establishment

Assay and protein target robustness testing

Assay screening qualification

Hit identification

True biophysical high-throughput screening

Screening with TRIC (MST), Spectral Shift, GCI, nanoDSF TSA

Library screening

Selected library subsets for focused screening

Competition and allosteric binding screenings

Protein-protein interaction (PPI) displacement screening

Screening of protein-macromolecule complexes (e.g. protein-RNA, protein-DNA)

Hit confirmation

Binding affinity via TRIC (MST), Spectral Shift, ITC

Binding kinetics via GCI

Label-free binding analysis via nanoDSF TSA

Thermodynamic profiling analysis via ITC

Hit characterization

Comprehensive in-solution direct binding with TRIC (MST) and Spectral Shift

Comprehensive in-solution, label-free direct binding with ITC

In-solution, label-free nanoDSF TSA

Comprehensive label-free kinetics binding with GCI

Ligand structural effect assays with SEC MALS and DLS

Target-aggregator assays with DLS

Hit optimization
Hit to Lead

Streamlining of suitable hit characterization assay

Regular application of streamlined assay in fragment evolution MedChem cycles

SAR support

What 2bind delivers

Fragment-based Drug Discovery: Use 2bind’s Biophysics Services for competitive timelines and throughput to promote your therapeutic program

Fragment-based Drug Discovery Services

In advance

During a project

Afterwards

Work with the experts in biophysics!