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2bind Services – Antibody Lead Selection

Antibody Lead Selection: A critical step supported by 2bind's biophysical solutions

Antibody Lead Selection

What 2bind can do for you
What 2bind delivers
In antibody lead selection, evaluating the developability of potential candidates is crucial for advancing promising therapeutic targets. At 2bind, we understand the significance of addressing the complexities inherent in antibody structures and their functional behavior. Our specialized biophysical services are uniquely equipped to assess the developability of antibody leads, providing accurate characterization and enabling informed decision-making in the pursuit of effective therapeutics.
  • Screen for binding with GCI, BLI (Grating Coupled Interferometry, Biolayer Interferometry)
  • Epitope Binning with BLI
  • Get binding kinetics (kon, koff, KD) with GCI, BLI, FPS (Fluorescence Proximity Sensing)
  • Get binding kinetics on live cells with RT-IC (Real Time Interaction Cytometry)
  • Get half maximal effective concentration (EC50) with Flow Cytometry

What 2bind can do for your antibody lead selection

Research and planning
Research and planning

Antibody team assembly

Antibody candidates analysis (A priori data analysis, structural analysis, …)

Target analysis (literature research, mechanistical research, etc.)

Selection of suitable technologies for lead ranking

Assay cascade definition

Access and sourcing of material

Assay establishment
Assay establishment

Protein target qualification

Technical lead selection assay establishment

Assay optimization

Assay screening qualification

Antibody lead selection screen
Antibody lead selection screen

Lead candidate ranking via label-free GCI and BLI

Lead candidate ranking via solution-based TRIC (MST) and Spectral Shift

Lead candidate ranking via cell-based Flow Cytometry

Candidate diversification via epitope binning with BLI

Lead candidates characterization
Lead candidates characterization

Antibody characterization via nanoDSF, DLS, HPLC

nanoDSF for conformational stability

DLS for colloidal stability and self-interaction propensity

SEC MALS for size determination and fragmentation, oligomerization and aggregation

HIC for hydrophobic profiling

IEX for charge profiling

Kinetics on live cells with RT-IC

Lead buffer optimization
Lead buffer optimization

Antibody developability studies

Pre-formulation of selected lead candidates

What 2bind delivers

In advance

  • Detailed technical and scientific project proposal
  • Detailed project milestones and timelines
  • Detailed cost structure

During a project

  • Frequent progress updates
  • Detailed and actionable milestone reports
  • Face-to-face meetings at important decision points

Afterwards

  • Comprehensive project and milestone summary
  • Face-to-face wrap-up meetings
  • Scientific and technical consulting for follow-up steps
Did You Know?
FPS on the heliX® platform is an innovative method for binding kinetics, based on DNA nanolevers. Ideal for nucleic acids and proteins.

Work with the experts in biophysics!

Im Gewerbepark D19a
93059 Regensburg

+49 941 2000 0890
info@2bind.com

Get in Touch
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