Antibodies, especially bispecifics, present a unique challenge: their complex multidomain structures carry multiple potential liabilities. To unlock their full therapeutic potential, we believe in a meticulous, multiparameter characterization approach. This includes testing under both standard and stress conditions to ensure optimal performance.
Avidity effects from bivalency and bispecificity can significantly alter binding behavior compared to simpler 1:1 interactions. In the world of bispecifics, understanding these dual-targeting mechanisms and their intricacies is crucial for success.
At 2bind, our specialized biophysical services are designed precisely for this challenge. We unravel these complexities, providing precise solutions for binding dynamics and specificity, so you can make informed decisions with confidence. Let our expertise guide you towards the successful development of your therapeutics.
Antibody team assembly
Antibody analysis (A priori data analysis, structural analysis, etc.)
Targets analysis (literature research, mechanistical research, etc.)
Selection of suitable technologies for binary and ternary interactions
Analytical and interaction assay cascade definition
Access and sourcing of material
Antibody and bispecifics qualification
Protein targets and reagent qualification
Technical assay parameter establishment for selected technologies
Analytical and interaction assay optimization
nanoDSF for conformational stability and high-throughput stability screens
DLS for colloidal stability and self-interaction propensity and high-throughput stability screens
SEC MALS for size determination and fragmentation, oligomerization and aggregation
HIC for hydrophobic profiling
IEX for charge profiling
Label-free kinetic binding GCI assays for high-throughput affinity/avidity screens
Label-free kinetic binding BLI assays for affinity/avidity screens in complex matrices, and epitope binning
Kinetics on live cells with Flow Cytometry and RT-IC
In-solution direct binding TRIC (MST), Spectral Shift assays
In-solution, label-free ITC assays for thermodynamic profiling and stoichiometry analysis
Dual color FPS assay for ternary complex analysis and avidity screens
Flow cytometry for high-throughput screens for cell binding
What 2bind delivers for Antibody characterization
