Work with the Fastest CRO in the World and Unlock the Potential of Your Small Molecule Drug Discovery
Stand-Alone Services for Therapeutic Small Molecules.
Our stand-alone offering includes access to quality proteins, high-throughput interaction screening, direct binding assessments, functional cell-based or enzymatic assays, and physicochemical and stability profiling. These services are backed by our scalable assays and rapid turnaround times, ensuring reliable and actionable data.
With deep experience across diverse chemical modalities and target classes, our scientific team delivers fit-for-purpose solutions that integrate seamlessly into your project – whether you’re at hit discovery, optimization, or any point in between.
Our Stand-Alone Services for your Small Molecule Drug Discovery
Small scale protein target purification
Small scale protein target purification
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Construct Design Consulting or Access to Catalogue Proteins
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Production Consulting
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Purification Monitoring
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Quality Controlling
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Direct Binding Assays
Direct Binding Assays
Affinity Determination
Spectral Shift, TRIC (MST), ITC, FP Assay, Flow Cytometry
Kinetics Determination
GCI (SPR-like)
Covalent Binding Assessment
Spectral Shift, GCI/SPR
Stoichiometry Determination
ITC, Spectral Shift, TRIC, GCI/SPR
Interaction Thermodynamics
ITC
Thermal Shift Assay
nanoDSF
Screening of Small Sets or Libraries
GCI/SPR, Spectral Shift, nanoDSF TSA, FP Assay
Ternary Complex Assay Formats
GCI, Spectral Shift, TRIC, BLI, nanoDSF, DLS
Functional assays
Functional assays
Cell Viability and Toxicity (Cell Death)
BSL1, BSL2, state-of-the-art kits
Cellular Signaling & Cellular Response
BSL1, BSL2, state-of-the-art kits, direct or second messenger (e.g. GPCR)
Target Engagement
BSL1, BSL2, NanoBRET®, state-of-the-art kits
Enzyme Kinetics
State-of-the-art kits, Kinases, Phosphatases, etc.
Physicochemical and Stability Assessments
Physicochemical and Stability Assessments
Small Molecule Aggregation and Solubility
DLS
Target Conformational and Colloidal Stability
nanoDSF, DLS
Target Size and Oligomerization
SEC-MALS, HPLC
Target Aggregator Assays for Binding Analysis
nanoDSF, DLS, SEC-MALS, HPLC
Target and Lead Stability Optimization Approach
nanoDSF, DLS, SEC-MALS, HPLC, binding assay
Other services
Other services
Small Molecule Library Spotting
Compound library management
Small Molecule Cherry Picking and Shipping
Compound library management
Platforms and standing assays
Platforms and standing assays
Target Feasibility Platform
Explore whether your target construct is suitable for a relevant binding assay within two weeks
Standing Binding Assays
Reach out to us if you want to know if we have a standing assay for your target construct
Direct Binding Data Generation for AI-based Drug Discovery
Artificial intelligence is transforming drug discovery by generating vast numbers of virtual compounds and in silico predictions. At 2bind, we close the gap between digital design and experimental reality with direct binding screening and validation services tailored for AI-driven approaches.
Whether you need to screen a large in silico library, test a few hundred compounds, or validate selected virtual hits, our scalable biophysical assay platform delivers rapid, high-quality data on binding affinity, kinetics, and specificity. Nothing provides deeper insights than biophysics – and with our data, your algorithms don’t just get validated, they get smarter. By feeding high-resolution binding data back into your AI models, we help refine and train algorithms to improve future predictions.
We also support validation runs for virtually designed small molecules, ensuring that promising candidates are confirmed early and that AI-generated hypotheses are tested against real-world molecular interactions.
With 2bind as your partner, you gain the reliability, speed, and scalability needed to integrate AI-based discovery seamlessly into your drug development pipeline.
Get in Touch!
"We worked with the 2bind team for several years and continue to be impressed by their exceptional scientific expertise and experimental capabilities, their high flexibility regarding timelines and a great enthusiasm to explore out of the box assays. All this is paired with the team’s kind and friendly spirit, making them a real pleasure to work with."
Patrick Kunz, PhD, Director of Experimental Biophysics
We Commit to Rapid Timelines
High Quality Molecules for High Precision Analytics
Small-Scale Protein Target Purification
High-quality binding assays and screening campaigns depend entirely on the structural integrity and purity of the target protein. At 2bind, our protein production service is fully tailored to generate assay-ready targets optimized for downstream characterization. We cover the entire small-scale pipeline from construct design to rigorous quality control, utilizing both prokaryotic and eukaryotic expression systems to match your target’s complexity.
Our specialized purification platform includes:
E. coli Expression Systems: Efficient and robust production of soluble, high-yield bacterial proteins and domains.
HEK293 Mammalian Expression: High-end transient expression in mammalian HEK cells to ensure correct post-translational modifications, proper folding, and native functionality of complex human targets.
Construct & Protocol Optimization: Custom engineering of tags, promoters, and expression conditions to overcome challenging solubility or stability bottlenecks.
Rigorous Quality Control: Every batch undergoes strict analytical verification (including purity, aggregation status) to guarantee reproducible, artifact-free binding data.
By bridging the gap between molecular biology and biophysics, we deliver tailored protein samples that are perfectly optimized for your specific screening and interaction analysis workflows.
Purified Protein, Characaterization and HTS
Binding, Kinetics, Affinity, Avidity
Characterizing the interaction between a small molecule and its target is our core expertise. We deliver high-resolution binding datasets with extraordinarily fast turnaround times, providing the rapid, reliable feedback loops essential for modern drug discovery. Our platform is fully scalable: we seamlessly support everything from high-throughput primary screens for broad hit identification to low-throughput deep dives for precise characterization.
By matching the right technology to your project’s scale, we actively drive and de-risks every critical phase of your early drug discovery pipeline:
Hit Identification & Validation: Rapidly screening hundreds to thousands of small molecules and peptides to identify true binders, successfully weeding out false positives early on.
Hit-to-Lead & Lead Optimization: Delivering thermodynamics, affinity, and kinetic resolution needed to understand structure-activity relationship (SAR) and guide medicinal chemistry.
Our scalable assay platform includes:
Broad Modality Compatibility: Our assay platform is universally compatible with a vast range of chemical modalities, including classic Small Molecules, Compounds, Fragments, PROTACs / Molecular Glues, RIPTACs, Peptides, Covalent Small Molecules, Macrocycles and more.
Affinity & Stoichiometry (Kd): High-throughput or deep-dive quantification of binding strengths and stoichiometries utilizing Spectral Shift, TRIC (MST), FP (Fluorescence Polarization), and ITC.
Real-Time Kinetics (kon/koff): High-resolution kinetic profiling via GCI/SPR (Grating-Coupled Interferometry) for maximized speed, throughput, and sample efficiency.
Covalent Binding & Thermodynamics: Advanced characterization of irreversible binders via orthogonal GCI/Spectral Shift assays (kinact/ki), and full thermodynamic profiling (Delta H, Delta S) via ITC.
- Direct Binding: Our general scalable assay platform comprises Spectral Shift, TRIC (MST), ITC, FP, Flow Cytometry, GCI, thermal shift assay (TSA), DLS and more for binary interactions, ternary formats (e.g. PPIs), HTS campaigns and orthogonal validations.
Target Cells, Function, and More
Functional Assays
To seamlessly bridge the gap between initial hits and biological reality and relevance, we offer customized functional workflows. We operate in state-of-the-art BSL1 and BSL2 (Biosafety Level 1 & 2) laboratories, allowing us to safely handle a vast range of standard cell lines, primary cells, and complex cellular models to translate binding into functional biological readouts.
Our functional testing platform focuses on:
Cell Viability and Toxicity (Cell Death): Quantifying compound safety, cytotoxicity profiles, and cell death dynamics (apoptosis/necrosis).
Cellular Signaling & Cellular Response: Tracking downstream phenotypic changes, secondary messenger cascades (e.g. for GPCR targets), and other cellular responses.
Target Engagement: Verifying that your small molecule successfully enters the cellular environment, crosses the cell membrane, and binds its intended target inside living cells.
Enzyme Kinetics: Performing precise, cell-free or cell-based enzyme kinetics profiling and IC50/EC50 determinations to characterize enzyme modulation, utilizing advanced readouts like absorbance, fluorescence, or luminescence.
Stability, Solubility, Aggregation and More
Physicochemical and Stability Assessments
Potency means nothing if a compound fails due to poor solubility, or if the target protein lacks the necessary structural integrity. At 2bind, we deploy high-end biophysical characterization to evaluate small molecule/peptide solubility and soluble aggregation and protein stability, protecting your campaign from misleading artifacts and ensuring smooth downstream development.
Our characterization platform delivers detailed insights across five core approaches:
Small Molecule Aggregation & Solubility: Mapping maximum compound solubility boundaries across physiological buffers and DMSO-tolerability ranges, while actively screening for aggregation and precipitation using DLS (Dynamic Light Scattering).
Target Conformational & Colloidal Stability: Assessing the structural robustness, thermal unfolding profiles (Tm, Tagg, Ton), and temperature-induced aggregation tendencies of your target proteins using orthogonal nanoDSF and DLS readouts.
Target Size & Oligomerization: Determining the precise absolute molecular weight, oligomeric state, and monodispersity of your protein targets in solution via SEC-MALS (Size Exclusion Chromatography paired with Multi-Angle Light Scattering).
Target Aggregator Assays for Binding Analysis: Validating whether hits are true, well-behaved chemical leads or non-specific, artifact-inducing aggregators by utilizing an advanced combination of nanoDSF, DLS, and SEC-MALS.
Target & Lead Stability Optimization Approach: Actively guiding development and optimization of buffer conditions to stabilitze tricky target proteins and compounds using nanoDSF, DLS, SEC-MALS, and direct binding assays.
Our Target Feasibility Platform
In early-stage drug discovery, speed is critical. Our Target Feasibility Platform is designed to quickly determine whether a target construct is suitable for a relevant biophysical binding assay. By combining streamlined workflows with our specialized expertise, we deliver reliable results within just two weeks – allowing you to move forward with confidence and without delay.
This rapid turnaround empowers project teams to make informed decisions early, saving valuable time and resources, and ensuring that only the most suitable targets enter the next stage of development. We have built this platform with the specific goal of accelerating the discovery process – because in drug discovery, every day counts.
So if you are working with platforms that produce multiple targets, target constructs, make sure to contact us for our target feasibility platform.
Even for single target companies, we apply our target feasibility platform to quickly identify applicability.
Kinetics Determination Assay
Affinity Determination Assay
Qualitative Binding Assay
Thermodynamic Binding Assay
Our Suite of Standing, Scalable Binding Assays – Ready to Accelerate Your Discovery Phase
Drawing on years of experience in drug discovery, we have developed a suite of pre-developed, high-quality binding assays for a wide range of protein targets. These assays are ready to deploy after a feasibility check, enabling rapid screening of small molecules without the need for assay establishment.
Each standing assay includes reliable production and sourcing of high-quality molecules, ensuring that your screening campaign starts on solid ground. Our assays are fully scalable—from small exploratory screens to high-throughput campaigns—providing flexibility to match your project needs and timelines. By combining proven assay designs with robust infrastructure and quality control, we deliver reproducible, reliable results that accelerate your path to hit identification.
Broad Expertise Across Chemical Modalities and Target Classes
Our small molecule services are designed to support all chemical modalities — from fragments to complex small molecules and covalent modalities.
Our team of experts combined with our biophysical platform brings extensive experience and value across a range of target classes, including enzymes, receptors, protein–protein interactions, or RNAs. Whether your molecule is classical or intrinsically disordered, we provide the processes and capabilities to move your project forward with confidence.
Chemical Modalities
- Compounds
- Fragments
- Covalent small molecules
- PROTACs, Glues, RIPTAcs, other multispecifics
- Peptides
- Macrocycles
Target Classes
- Globular Proteins
- Intrinsically Disordered (IDPs, IDRs)
- Membrane Proteins
- Protein-Protein Interactions (PPIs)
- Transkription Factors
- RNA, DNA and Aptamers
Disease Areas
- Cancer
- Autoimmune diseases
- Infectious diseases
- Neurological disorders
- Metabolic diseases
- Cardiovascular diseases
Get in Touch!
"Their science and professionalism are impeccable – working with 2bind is like having experts in the lab next door."
Chris Lepre, PhD, Relay Therapeutics
Get In Touch
Planning a research project or interested in our analytical services? Get in touch with our team to discuss your requirements.
- +49 941 2000 0890
- info@2bind.com
- Im Gewerbepark D19a, 93059 Regensburg, Germany