Deciding on the final lead out of a pool of promising candidates can be a challenge and the better the candidates the more the decision makers will be spoilt for choice. At this stage we recommend extending the characterization and including methods that are higher in material consumption but deliver details that cannot be resolved in the high throughput methods. Furthermore, we suggest keeping an eye on the physiological situation and assessing affinities under avidity-allowing and avidity-excluding conditions plus analyzing real-time kinetics of target cell binding – if of relevance.

At this important stage of the project, we implement off-target screens to flag potential issues which are easily overlooked and can cause costly failures in the clinic. We are equipped with a BSL2 cell culture lab and cell based functional assays can be implemented according to the project’s requirements.