Work With the Most Trusted CRO and Proceed With Your Lead Antibody With Confidence
Stand-Alone Services for therapeutic Antibodies.
Our offering includes studies to assess binding strength and specificity, ranging from high throughput binding screens applying multiple target proteins, affinity ranking, species-cross reactivity studies, comprehensive evaluation of kinetic interactions with purified target proteins and target cells, as well as developability assessments, preformulation studies and storage stability studies.
These services are powered by our scientific expertise and experience in the field, our high-end equipment enabling fast turnaround times, ensuring high-quality, reproducible data, and our responsive and transparent project management with a strong customer-first mindset.
With extensive experience across antibody modalities and target types, our scientific team creates fit-for-purpose solutions that support your project – whether you are in the screening phase, in the lead selection process, or are preparing for clinical advancement.
Our Stand-Alone Solutions for Your Therapeutic Antibody Projects
Target binding: Kinetics & Affinity
Target binding: Kinetics & Affinity
Binding screens with purified protein and cells
Affinity ranking
GCI, BLI, Flow Cytometry, KinExA, strong affinity
Kinetics of target and target cell binding
heliXcyto, scIC, RT-IC, avidity
Functional Characterization
Functional Characterization
Ligand inhibition
Flow Cytometry, heliXcyto, receptor occupancy assay
NK/T-Cell recruiting assay
TCEs, BSL1, BSL2, state-of-the-art kits
Signal induction
second messenger, state-of-the-art kits
Cell killing
ADCC, CDC
Internalization
Direct labeling, Fab-based labeling
Physicochemical and Stability Assessments
Physicochemical and Stability Assessments
Multiparametric unfolding and aggregation studies
DLS, nanoDSF
Conformational/thermal and colloidal stability (Ton, Tm, Tagg)
DLS, nanoDSF
Self-interaction assessment
DLS
Low molecular (LMW) and high molecular weight (HMW) content determination
SEC MALS, HPLC, DLS
Hydrophobicity profiling
HIC, HPLC
Charge heterogeneity determination
IEX, HPLC
Supporting Services
Supporting Services
Receptor quantification assay
BSL1, BSL2, Flow Cytometry
Short-, mid-, long-termin stablity assessment
Accelerated stability studies, stress studies, thermal, colloidal, mechanical, oxidative, chemical stability
Stability optimization via Design of Experiment
Pre-formulation, DSD, RSM, DoE
Target protein deconvolution
Protein array, ELISA
FcR and FcRn affinities
BLI, GCI, Spectral Shift, TRIC
Developability assessments
Stability, binding, affinity, kinetics, State-of-the-art kits, ELISA, polyreactivity, hydrophobicity, self-interaction, viscosimetry
Off-target binding assessment
State-of-the-art kit, ELISA
Scouting for stabilizing buffers
Explore our off-the-shelf solution or highly customizable approach
Unparalleled Precision at the Molecular Level
Multiparametric Stability Analysis
To analyze conformational and colloidal stabilities, we monitor thermally induced unfolding, changes in the hydrodynamic radius and the formation of small and large aggregates, delivering Ton, Tm and Tagg with extraordinary precision. The method detects tiniest changes in the sample and is ideal for monitoring sample quality during storage, freeze-thaw cycles, or other treatments.
Reliable and reproducible thermal stability parameters, measured with just 10 µg of protein, provide important information when it comes to ranking of multiple candidates.
Purified Protein and Target Cells
Binding, Kinetics, Affinity
At 2bind we know that kinetics and affinities can be determined in multiple ways, each way with a different focus, and ideally complementing each other.
We perform binding studies with purified recombinant protein and target cells adjusting the setup to your project requirements. If you need a screen aiming for a yes/no answer for different antibodies assessing binding to one target protein, or one antibody screened for binding to a panel of proteins, or anything in between – we plan the project according to your needs. Detailed kinetic measurements in avidity-excluding or avidity-allowing setups are possible with single antibodies or 100s of candidates. In addition to studying kinetics with purified protein, we can measure real-time kinetics with living cells, adding the complexity of the semifluidic and crowded membrane surroundings to the interaction equation.
For soluble targets in contrary, the physiological situation is better reflected by methods that determine interaction affinities having both binding partners in solution. We set up our technology platform to have choices for real-time kinetics with purified target protein and target-expressing cells as well as methods to determine in-solution affinities.
Assess the Soft Skills of Your Therapeutic Candidates
Developability
Binding to its target within the envisioned affinity window and showing the functionality that is required to reach the therapeutic effect, are certainly prerequisites for a candidate to succeed in the clinic. However, a successful candidate needs to be stable during storage and handling, highly specific for its target protein, cross-reactive to the orthologue protein in the relevant species, non-immunogenic, showing ideal biodistribution and tissue penetration as well as having ideal pharmacokinetic properties.
Many of these properties can be addressed directly within the screening cascade – or were shown to correlate with parameters that can be measured directly.
Developability screens assess the “soft skills” of antibodies. Not all candidates with developability liabilities will fail in clinical development, but some liabilities are indicative for an increased risk of failure. If recognized, these risks can be mitigated by sequence engineering, formulation and storage conditions.
Sometimes We Need High Performance
Size, hydrophobicity, charge heterogeneity
Some scientific questions need High Performance Liquid Chromatography to be answered.
We have developed several chromatography methods for checking molecular weights of proteins or protein complexes, measuring HMW and LMW content of protein preparations, and comparing hydrophobicity and charge heterogeneity of sequence variants or samples prepared or stored under different conditions.
We use HPLC methods to support screens or rankings of different candidates, deliver additional read-out parameters in storage stability studies, or in customized applications.
Our dedicated Preformulation Services
Buffer conditions don’t just complement the amino acid sequence, they are the unsung heroes shaping an antibody’s physicochemical properties, molecular integrity, and storage stability. By fine-tuning thermal resilience, curbing aggregation, and dialing down viscosity with smart excipients, we eliminate risks in sample shipment and storage for your preclinical and clinical studies.
As antibody-based drugs evolve into complex bi- and multispecific structures, fusions with non-antibody proteins add new functionalities, and high-concentration formulations have been trending for years, formulation optimization is no longer optional; it’s mission-critical.
At 2bind, we’re obsessed with finding your molecule’s ideal conditions. Dive into our AI-crafted Signature Buffers, born from evaluating 161 approved therapeutic antibody drugs. It’s like a chef’s tasting menu for proteins – meet the stars and discover your molecule’s true love:
SweetHarmony
For those who thrive in sugary bliss.
SaltyDream
When a briny embrace hits just right.
SalineBased
The reliable classic, no frills needed.
SuperSweet
Extra indulgence for the bold.
SourSalt / SourSweet
Because sometimes opposites really attract.
SkilledGues
Our ace in the hole when others fall short.
Beyond the menu, our Quick ‘n’ Tidy buffer screen systematically probes pH variations paired with salts, sugars, sugar alcohols, amino acids, and detergents to map your molecule’s sweet spot. Struggling with viscosity capping concentrations? Our Fluidifiers push the limits. For tailored challenges, customized DoEs with appropriate read-out parameters deliver formulations that solve your specific problem.
Multiparametric Stability Analysis
Self-interaction and viscosity
Size Distribution, Charge heterogeneity
Broad Expertise Across Antibody Modalities, Target Types and Disease Areas
Our antibody development services cover the full spectrum of therapeutic antibody modalities: from canonical full length IgGs and bispecific formats to antibody fragments and antibody-fusion proteins.
Leveraging our high-end equipment and deep scientific expertise, we work across diverse target types, including membrane proteins, soluble proteins, and complex multiprotein systems.
Whether your antibody is conventional or engineered for unique functions, we provide the processes, data quality, and speed to confidently advance your program.
Antibody Modalities
- IgG1, IgG4, Fc-silenced IgG
- Bispecifics
- Multispecifics
- Nanobodies
- scFvs
- Fabs
- Antibody-fusion proteins
- ADCs
Target types
- Membrane proteins
- Soluble proteins
- Multiprotein complexes
- Target cells
Disease Areas
- Cancer
- Autoimmune diseases
- Infectious diseases
- Neurological disorders
- Metabolic diseases
- Cardiovascular diseases
Get in Touch!
"I’ve always been extremely satisfied with the service provided by 2bind. Communication has consistently been straightforward and uncomplicated, making collaborations very pleasant and efficient. The results and data we received were of high quality and fully trustworthy, which gave us great confidence in our decision-making. I happily recommend 2bind to anyone looking for reliable and professional support in their projects."
Johanna Gellert, PhD, Pentixapharm
Get In Touch
Planning a research project or interested in our analytical services? Get in touch with our team to discuss your requirements.
- +49 941 2000 0890
- info@2bind.com
- Im Gewerbepark D19a, 93059 Regensburg, Germany